银杏叶提取物EGb761对NO供体硝普钠诱导的海马神经元凋亡的保护作用  

Protective effects of extract of Ginkgo biloba leaves ( EGb 761) on apoptosis of cultured hippocampal neurons induced by nitric oxide donor, SNP

作　　者：张海风[1] 刘勇军[1] 祝其锋[1] 张海涛[1] 

机构地区：[1]广东医学院生物化学与分子生物学研究所,广东湛江524023

出　　处：《中国现代应用药学》2003年第2期94-97,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：广东省重点学科重点项目资助课题 (№ 980 8)

摘　　要：目的　探讨银杏叶提取物 (EGb 761 )对NO供体硝普钠 (SNP)引起的大鼠海马神经元凋亡的影响。方法　采用MTT比色分析测细胞存活率、Hoechst 332 58荧光染色及DNA琼脂糖凝胶电泳分析等方法检测凋亡。结果　不同剂量EGb761预处理海马神经元 6h可剂量依赖地对抗SNP引起的神经元凋亡 ,提高神经元的存活率 ;减少SNP引起的核固缩、凝聚和碎裂现象 ;DNA凝胶电泳图谱未见典型的“梯子状”改变。结论　EGbOBJECTIVE To study the effects of Extract of Ginkgo biloba leaves ( EGb 761) on apoptosis of cultured hippocampal neurons induced by nitric oxide donor, sodium nitroprusside (SNP). METHOD Neuronal viability and morphology were tested by MTT assay and light microscope. Fluorescent staining with Hoechst 33258 and agarose gel electrophoresis was used to analyze apoptosis.RESULTS Pretreatment with different concentrations of EGb 761 (7.5～90μg/mL) for 6h increased the survival rate of neurons in a concentration dependent manner. EGb 761 prevented the neuronal nuclei from shrinkage, condensation and cleavage induced by SNP. It also blocked neuronal nuclear DNA fragmentation induced by SNP.CONCLUSION These results indicated that EGb 761 can protect neurons from apoptosis induced by NO.

关 键 词：阿尔茨海默病 银杏叶提取物 神经元凋亡 硝普钠 MTT比色分析法 Hoechst33258荧光染色 DNA琼脂糖凝胶电泳分析法 

分 类 号：R277.791.6[医药卫生—中医学]