GFP-hDaxx融合蛋白在巨噬细胞中的表达与定位  

The Expression and Localization of GFP-hDaxx Fusion Protein in Macrophages

作　　者：谭立志[1] 刘传爱[1] 刘安元[1] 万艳平[1] 

机构地区：[1]南华大学病原生物学研究所,中国湖南衡阳421001

出　　处：《实用预防医学》2003年第2期135-136,共2页Practical Preventive Medicine

基　　金：湖南省教育厅课题资助 (编号 :0 2C391)

摘　　要：目的　构建GFP -hDaxx融合蛋白真核表达载体pEGFP/hDaxx并在巨噬细胞中表达 ,为研究hDaxx对巨噬细胞凋亡及分泌活性的影响提供实验基础。　方法　将hDaxxcDNA片段亚克隆入载体pEGFP ,构建GFP -hDaxx融合蛋白真核表达载体。将质粒pEGFP/hDaxx转染巨噬细胞 ,Westernblotting鉴定表达产物 ,荧光显微镜观察GFP -hDaxx融合蛋白在细胞内的表达与定位。　结果　成功构建了GFP -hDaxx融合蛋白真核表达载体pEGFP/hDaxx ,GFP -hDaxx融合蛋白在巨噬细胞中表达并定位于细胞核。　结论　pEGFP/hDaxx在巨噬细胞中即时高表达GFPObjective To construct eukaryotic expression vector pEGFP/hDaxx, which can express GFP-hDaxx fusion protein, and to study the effect that hDaxx affects macrophages apoptosis and its secretory activities. Methods hDaxx was subcloned into pEGFP vector and positive colony was selected. pEGFP/hDaxx was transfected into macrophages. Expression product of GFP-hDaxx fusion protein was analyzed by Western-blotting and observed localization under fluoscent microscope. Result pEGFP/hDaxx, which is an eukaryotic expression vector, was constructed successfully and GFP-hDaxx fusion protein were expressed ideally in macrophages and localized in nuclei. Conclusion GFP-hDaxx fusion protein immediately expressed in macrophages successfully and localized in nuclei.

关 键 词：HDAXX 巨噬细胞 表达 定位 

分 类 号：R37[医药卫生—病原生物学]