小剂量电离辐射引起人淋巴细胞染色体的不分离  

EFFECT OF LOW DOSE IONIZING RADIATION ON HUMAN LYMPHOCYTES CHROMOSOME NON-DISJUNCTION

作　　者：李华[1] 刘雯[1] 李锦燕[1] 左伋[1] 

机构地区：[1]复旦大学上海医学院细胞与遗传医学系,上海200032

出　　处：《癌变．畸变．突变》2003年第2期67-71,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金资助项目(No.30100037)

摘　　要：目的 :探讨低剂量电离辐射引起人外周血染色体数目的变化及不分离情况。 方法 :用人外周血中期染色体制备并计数 ,用 χ2检验作统计学分析 ,检测在0、0.1、0.25、0.5、1.0、2.0Gyγ_射线照射后 ,不同时间给予0.5Gyγ_射线照射后及在接种前后给予不同次数0.5Gyγ_射线照射后非整倍体率及超二倍体/非整倍体变化。 结果 :在0～2.0Gy剂量范围内 ,非整倍体率随剂量增加从21.30 %增加到55.56 % ,超二倍体/非整倍体从8.70 %增加到44.00 % ;接种后照射比接种前照射非整倍体率及超二倍体/非整倍体高 ,且均比对照组高 ,在接种后不同时间照射的细胞无明显差别 ;在接种前照射 ,随照射次数增加到3次 ,非整倍体率从21.30 %增加到47.85 % ,超二倍体/非整倍从8.70 %增加到24.49 %。 结论 :小剂量电离辐射对非整倍体特别是染色体不分离的影响具有剂量效应、次数效应 。Purpose:To explore the changes of chromosome number and chromosome non_disjunction in human lymphocytes treated with low dose ionizing radiation. Methods: The changes of chromosome number were measured by metaphase chromosome counts, and statistic an analysed with χ 2 test. Results: After treated whole blood with 0, 0.1, 0.25, 0.5, 1.0, 2.0 Gy γ_rays, aneuploidy rate increased from 21.30 % to 55.56 % as dose increased, so did hyperdiploid / aneuploid, which increased from 8.70 % to 44.00 %. After treated with 0.5 Gy at different time, the two targets were significantly higher than control(P<0.01), and the degree of increasing of the culture radiated was higher than that of the whole blood radiated. When the culture was radiated,the two targets had no difference in different radiation time. When the whole blood was radiatied, the two targets had the tendency to increase as the radiation times increased. Conclusion: The effect of low dose γ_rays on aneuploid, especially chromosome non_disjunction is dose_dependent and time_dependent. And the effect is outstanding when the culture radiated than when the whole blood was radiated.

关 键 词：小剂量电离辐射 人淋巴细胞 非整倍体 染色体不分离 

分 类 号：R818.03[医药卫生—放射医学]