表面等离激元共振(SPR)生物传感器对聚氨酯材料血浆蛋白吸附的实时原位动态研究  

USE OF SURFACE PLASMON RESONACE(SPR) SENSOR FOR REAL TIME IN SITU STUDY OF ADSORPTION OF PROTEINS ONTO SURFACE OF POLYURETHANE

作　　者：吕晓迎[1] 黄炎[1] 唐祖明[1] 洪庆月[1] 陆祖宏[1] 钟银屏[2] 叶卫华[1] 刘凌云[1] 

机构地区：[1]东南大学生物医学工程系吴健雄实验室,南京210096 [2]四川大学高分子材料系,成都610065

出　　处：《中国生物医学工程学报》2003年第2期165-170,164,共7页Chinese Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目 (60 12 110 1;3 0 2 73 91) ;"863"资助项目 (2 0 0 2AA3 2 60 64 )

摘　　要：应用实验室自行研制的自动扫描式表面激元共振 (SPR)生物传感器对三种聚氨酯材料进行了血液蛋白质吸附实验 ,以传感片上的金膜作为对照材料。同时应用原子力显微镜对金膜和聚氨酯材料的超微结构与材料表面上所吸附的蛋白质进行了表征。实验结果显示 ,四种材料对纤维蛋白原和IgG的吸附量顺序均为 :金膜 >H5 0 0 >H5 0 5 0 >H5 0 10 0。T 检验结果表明 ,金膜对纤维蛋白原和IgG吸附量与三种聚氨酯材料均有显著差别。该结果表明聚氨酯材料的血液相容性明显好于金膜对照材料。The kinetic behavior of human fibrinogen (Fib) and human IgG adsorbed onto surfaces of polyurethane (PU) H50-0, H50-50, H50-100 and gold film has been investigated by using an SPR sensor. The surface of the gold film, the microstructure of the polyurethane film and the surfaces with adsorbed proteins were imaged with an atomic force microscope (AFM). The SPR results showed that the adsorbed fibrinogen and IgG on the surfaces of materials is of the sequence: gold film > H50-0 > H50-50 > H50-100. T-test of both SPR results indicated that the differences of adsorbed fibrinogen and IgG on gold film and polyurethane are significant, which demonstrate that the blood compatibility of polyurethane is better than that of the gold film.

关 键 词：表面等离激元共振 生物传感器 聚氨酯材料 血浆 蛋白吸附 实时原位动态研究 

分 类 号：R318.08[医药卫生—生物医学工程]