噻二唑苯基脲在平贝母脱分化过程中引起的生理生化变化  

Thidiazuron induced physiological and biochemical changes in the process of dedifferentiation of Fritillaria ussuriensis

作　　者：刘亚菊[1] 殷红[2] 朱四易[2] 

机构地区：[1]江苏徐州甘薯研究中心国家甘薯改良中心,江苏徐州221121 [2]西北大学生命科学学院,西安710069

出　　处：《西北植物学报》2003年第3期433-437,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(37170097)

摘　　要：平贝母(Fritillaria ussuriensis Maxim)鳞茎切块分别在含噻二唑苯基脲(TDZ)或激动素(Kin)的培养基上脱分化过程中的苯丙氨酸解氨酶、淀粉酶、吲哚乙酸氧化酶和过氧化物酶活性均增强,在外植体脱分化前达到峰值,随后下降。TDZ处理下这些酶的活性均高于Kin处理。可溶性蛋白含量的变化趋势与酶活性变化相同,只是TDZ作用下蛋白含量增加的相对水平较低。说明TDZ具有比Kin更强的细胞分裂素活性。Some physiological and biochemical changes in the process of dedifferentiation of F. ussuriensis bulb cuttings cultured on the media containing thidiazuron (TDZ) or kinetin (Kin) had been studied. Re-sults showed that phenylalanine ammonia lyase,amylase,indoleacetic acid oxidase,and peroxidase activities and soluble protein content increased in the process of dedifferentiation,and reached the peak value before callusing,then decreased. The activities of these enzymes under TDZ treatment were higher than that under Kin,while soluble protein content under TDZ was lower than that under Kin.

关 键 词：平贝母 脱分化过程 噻二唑苯基脲 培养基 丙氨酸解氨酶 淀粉酶 吲哚乙酸氧化酶 过氧化物酶 酶活性 可溶性蛋白含量 组织培养 细胞分裂素 

分 类 号：S567.231[农业科学—中草药栽培] S336[农业科学—作物学]