补硒与锌对梭曼中毒大鼠乙酰胆碱酯酶活力和抗氧化力的影响  

Effect of Se and Zn on acetylcholinesterase and antioxidative capacity in soman poisoned rats

作　　者：杨兴斌[1] 杨会宣[1] 蒋宁[1] 赵燕[2] 谷小刚[1] 海春旭[1] 

机构地区：[1]第四军医大学毒理学教研室,陕西西安710032 [2]第四军医大学化学教研室,陕西西安710032

出　　处：《中国药理学与毒理学杂志》2003年第2期117-120,共4页Chinese Journal of Pharmacology and Toxicology

基　　金：全军"十五"重点科研资助项目 (0 1L0 77)

摘　　要：目的　探讨抗氧化剂硒 (Se)和锌 (Zn)预防梭曼中毒的可能性。方法　雄性大鼠 4 0只 ,按体重随机分为正常对照组、染毒组、硒治疗组、锌治疗组。正常对照组和染毒组每日 5mL·kg- 1的生理盐水(NS)ig ;硒治疗组每日 1mg·kg- 1Na2 Se0 3的NS液ig和锌治疗组每日 4mg·kg- 1Zn(C6 H11O7) 2 的NS液ig ,共ig 9d。d 10除正常对照组外其余各组大鼠均sc 0 .9LD50 梭曼 ,2h后断头处死并取样 ,测定大鼠全血乙酰胆碱酯酶 (AChE)活性及血清、大脑、肝脏的超氧化物歧化酶 (SOD)活性、总抗氧化力 (T AOC)和一氧化氮合酶 (NOS)活性。结果　梭曼中毒大鼠后 ,染毒组大鼠全血AChE活性被明显抑制 ,血清、大脑、肝脏的SOD活性、T AOC显著下降 ,NOS含量明显升高 ,而硒和锌预防组大鼠对梭曼引起的AChE、SOD、NOS活性和T AOC变化明显减轻。AIM To study the feasibility of prevention from soman intoxication by antioxidants Se and Zn. METHODS Forty male rats were randomly divided into 4 groups: control group, soman group, Se group, and Zn group. Rats in control and soman groups were orally administrated with 5 mL·kg -1 physiological saline per day for 9 d. Rats in Se group received 1 mg·kg -1 Na 2SeO 3 and rats in Zn one received 4 mg·kg -1 zinc gluconate. Except control group, rats were given sc 0.9 LD 50 soman on the 10th day. After 2 h, the rats were sacrificed. Acetylcholinesterase(AChE) of whole blood and superoxide dismutase(SOD), nitric oxide synthase(NOS) activity and total antioxidative capacity(T AOC) of soman poisoned rat serum, cerebrum and liver were determined respectively. RESULTS AChE, SOD activity and T AOC of rats decreased and NOS activity increased at 2 h after sc soman. Pretreatment of Se and Zn reduced the changes in AChE, SOD, NOS activity and T AOC induced by soman. CONCLUSION Se and Zn have preventive effect against soman intoxication in rats.

关 键 词：梭曼中毒 硒 锌 乙酰胆碱酯酶 超氧化物歧化酶 总抗氧化力 一氧化氮合酶 

分 类 号：R595[医药卫生—内科学] R979.3[医药卫生—临床医学]