头颈鳞癌血管生成与颈淋巴结转移相关性研究  

Study on the relationship between angiogenesis and cervical lymphatic metastasis in head and neck squamous cell carcinomas

作　　者：郑海洲[1] 林山[1] 

机构地区：[1]浙江大学医学院附属第一医院耳鼻咽喉科

出　　处：《临床耳鼻咽喉科杂志》2003年第5期288-290,共3页Journal of Clinical Otorhinolaryngology

摘　　要：目的 :探讨头颈鳞癌血管生成与其颈淋巴结转移的关系以及血管内皮生长因子(VEGF)在头颈鳞癌血管生成中的作用。方法 :应用免疫组化SABC法检测 5 8例头颈鳞癌组织中微血管密度 (IMVD)及VEGF的表达。结果 :5 8例头颈鳞癌组织中IMVD为 2 3.93± 8.77,肿瘤分化程度 ,高与中、高与低间 ,IMVD差异有显著性意义 (均P <0 .0 5 ) ;中与低间 ,差异无显著性意义 (P >0 .0 5 )。颈淋巴结转移组IMVD(2 7.92± 9.11)明显高于非转移组 (2 0 .6 9± 7.0 8) ,其差异有显著性意义 (P <0 .0 1)。癌组织中VEGF表达与瘤内IMVD呈正相关 (rs=0 .4 87,P <0 .0 1)。结论 :瘤内IMVD可作为预测头颈鳞癌颈淋巴结转移的一个重要指标 ;VEGF可促进头颈鳞癌血管生成。Objective:To investigate the relationship between tumor angiogenesis and cervical lymphatic metastasis and the expression of vascular endothelial growth factor in head and neck squamous cell carcinomas.Method:Fifty-eight head and neck carcinoma tissue slides were immunohistochemically stained for anti-CD 34 monoclonal antibody and anti-VEGF polyclonal antibody by SABC method. The intratumor microvessel density (IMVD) and the expression of vascular endothelial growth factor were measured.Result:①The mean IMVD with cervical lymphatic metastasis was significantly higher than those without cervical lymphatic metastasis in head and neck squamous cell carcinomas( 27.92± 9.11 and 20.69± 7.08,P< 0.01);②The expression of vascular endothelial growth factor had a positive correlation with IMVD in head and neck carcinoma tissues (r s= 0.487,P< 0.01).Conclusion:IMVD may be an important predictor to cervical lymphatic metastasis in head and neck squamous cell carcinomas. Vascular endothelial growth factor may stimulate tumor angiogenesis in head and neck squamous cell carcinomas.

关 键 词：血管生成 血管内皮生长因子 头颈部肿瘤 颈淋巴结转移 癌 鳞状细胞 

分 类 号：Q58[生物学—生物化学]