西红花酸对异丙肾上腺素致大鼠急性心肌缺血损伤的保护作用  

Protective effect of crocetin on isoproterenol-induced myocardial injury in rats

作　　者：刘同征[1] 钱之玉[1] 

机构地区：[1]中国药科大学药理教研室,江苏南京210009

出　　处：《中草药》2003年第5期439-442,共4页Chinese Traditional and Herbal Drugs

摘　　要：目的　研究西红花酸对异丙肾上腺素 (ISO)致大鼠急性心肌缺血的保护作用及机制。方法　采用大剂量ISOsc诱导产生大鼠急性心肌缺血模型 (ISO 30mg/kg ,每日一次 ,连续 2d) ,实验大鼠随机分为正常组、ISO模型组、阳性对照组和 3个西红花酸给药组 (2 5 ,5 0 ,10 0mg/kg) ,测定心脏系数和血清中乳酸脱氢酶 (LDH)、磷酸肌酸激酶 (CK)和丙二醛 (MDA)含量 ,观察心肌组织谷胱甘肽过氧化物酶 (GSH Px)、Na+ ,K+ ATP酶和Ca2 + ,Mg2 + ATP酶活性及心脏病理改变 ,观察西红花酸的保护作用。结果　西红花酸能显著降低血清中CK和LDH的释放 ;也能显著降低血清和心脏匀浆中MDA水平 ,心脏重量参数显示对心肌水肿有明显的抑制作用 ;保护心肌GSH Px ;Na+ ,K+ ATP酶和Ca2 + ,Mg2 + ATP酶活性 ;心脏病理切片表明西红花酸对心肌坏死有显著的保护作用。结论　西红花酸对ISO所致急性心肌缺血有显著的保护作用。Object To study the protective effect of crocetin on myocardial ischemia induced by isoproterenol (ISO). Methods Myocardial ischemia was induced by subcutaneous injection of ISO (30 mg/kg for two days, once a day). The experimental animals were randomly divided into the normal group, ISO group, positive control group and three crocetin groups (25, 50, 100 mg/kg). Cardiac indexes were examined. The level of LDH, CK, MDA in serum were measured. The content of MDA, GSH-Px and the ATPase activity heart and mitochondria were assayed by colorimetric analysis. The histopathological change of myocardia was investigated by HE staining. Results Compared with the model group, crocetin can significantly reduce the cardiac indexes, obviously decrease the level of MDA, LDH and CK in the serum. Crocetin can significantly increase the activities of Na +, K +-ATPase; Ca 2+ , Mg 2+ -APTase; GSH-Px. The histopathological changes confirmed the protective effects of crocetin on the myocardial injury. Conclusion Crocetin could alleviate the acute myocardial ischemia induced by ISO in rats.

关 键 词：急性心肌缺血 西红花酸 异丙肾上腺素 大鼠 保护作用 

分 类 号：R285.5[医药卫生—中药学] R259.422[医药卫生—中医学]