反相高效液相色谱梯度洗脱同时测定复方茶碱片中7组分的含量  

Simultaneous Determination of Seven Ingredients in Compound Theophylline Tablets by Reversed Phase High Performance Liquid Chromatography Gradient Elution

作　　者：王霞[1,2] 刘道杰[1] 孙吉令[3] 

机构地区：[1]聊城大学化学系 [2]中国科学院兰州化学物理研究所,兰州730000 [3]山东省药品检验所

出　　处：《分析化学》2003年第5期577-579,共3页Chinese Journal of Analytical Chemistry

摘　　要：建立了一种灵敏、快速的反相高效液相色谱梯度洗脱方法,用于同时测定复方茶碱片中7组分的含量。采用YWG C18柱,以甲醇一磷酸二氢钠为流动相,用二极管阵列检测器,检测波长为214 nm,15 min内即可将7种组分分离测定。该方法快速准确,线性范围广,用于实际样品的测定,结果满意。A reversed-phase high performance liquid chromatography (RP-HPLC) gradient elution method was proposed for the simultaneous determination of seven active ingredients in compound theophylline tablets. A YWG C-18 column and a binary mobile phase of methanol and sodium dihydrogen phosphate were used. A photo-diode array detector was used to detect the compositions with 214 ran as the detection wavelength. The whole process could be performed within 15 minutes. The proposed method was accurate and rapid with wide linear range and reliable results. It has been applied to assaying the real sample with satisfactory results.

关 键 词：反相高效液相色谱 梯度洗脱 同时测定 复方茶碱片 药物含量 平喘药 

分 类 号：O657.72[理学—分析化学]