rhIL-11联合rhG-CSF动员小鼠外周血造血干/祖细胞的研究  

Study of murine hematopoietic stem/progenitor cell mobilized by recombinant human interleukin 11 combination with granulocyte-colony stimulating factor

作　　者：孟凡义[1] 蒋祖军[1] 易正山[1] 徐丹[1] 

机构地区：[1]第一军医大学南方医院血液科,广州510515

出　　处：《中华血液学杂志》2003年第5期225-227,共3页Chinese Journal of Hematology

基　　金：广东省自然科学基金重点资助项目 ( 2KM0 45 0 6S) ;广东省社会发展攻关项目 (B30 2 0 2 )

摘　　要：目的　研究rhIL 11对小鼠巨核系造血干 /祖细胞的动员作用。方法　rhIL 112 5 0μg·kg-1·d-1或联合rhG CSF 2 5 0 μg·kg-1·d-1给C5 7BL/ 6小鼠皮下注射 1～ 7d ,观察用药前和用药第 3,5 ,7,9天小鼠外周血白细胞、血小板计数 ,CD34 +细胞比例 ,CFU GM、CFU MK、CFU E的数量变化。结果　单用rhIL 11或与rhG CSF联合使用时 ,外周血白细胞、血小板、CD34 +细胞比例及各种造血细胞集落数明显高于对照组 (P <0 .0 1)。在含有IL 11的实验组中 ,CFU MK明显高于rhG CSF组 (P <0 .0 1)。结论　rhIL 11可升高外周血白细胞、血小板 ,同时增加外周血CD34 +细胞的比例 ,提高粒、红、巨核系造血细胞集落形成单位的数量 ,特别是对CFU MK作用较强 ;与rhG CSF联合使用对动员骨髓造血干 /祖细胞进入外周血有明显的协同作用。Objective To study the recombinant human interleukin 11(rhIL-11) in combination with granulocyte-colony stimulating factor (rhG-CSF) for mobilizing peripheral blood stem/progenitor cells in C57BL/6 mice. Methods rhIL-11 250 μg·kg -1·d -1 per mouse alone or in combination with rhG-CSF 250 μg·kg -1·d -1 per mouse was administered to C57BL/6 mice from day 1 to 7. The changes of peripheral white blood cell count(WBC), platelet counts(BPC) and hematopoietic stem/progenitor cells yields were observed. Results The results showed that rhIL-11 alone or in combination with rhG-CSF resulted in increase in absolute numbers of WBC, BPC, CD 34+ cells,and CFU-GM, CFU-E, CFU-MK yields in peripheral blood more than those of control (P<0.001). The yields of CFU-MK was significantly more than that of rhG-CSF group(P<0.001). Conclusion rhIL-11 alone or in combination with G-CSF could significantly mobilize hematopoietic stem/progenitor cells from bone marrow into peripheral blood.

关 键 词：白细胞介素-11 粒细胞集落刺激因子 造血干细胞 巨核细胞 

分 类 号：R457[医药卫生—治疗学]