IL-2和IL-15协同调节T细胞的增殖和LAK细胞的杀伤活性  

Synergistic effects of IL-2 and IL-15 on the proliferation of T cells and cytotoxicity of human LAK

作　　者：党娜娜[1] 范桂香[2] 朱勇[1] 刘雪松[1] 贾卫[1] 王薇[1] 袁育康[2] 金伯泉[1] 

机构地区：[1]第四军医大学免疫学教研室,陕西西安710032 [2]西安交通大学医学院免疫学教研室,陕西西安710062

出　　处：《免疫学杂志》2003年第3期175-177,182,共4页Immunological Journal

基　　金：高等学校骨干教师资助计划项目 (2 0 0 0 - 6 5 - 6 6 )

摘　　要：目的　探讨IL 2和IL 1 5在免疫调控和应答中的协同作用。方法　IL 2、IL 1 5和IL 2 +IL 1 5组刺激CTLL细胞的增殖 ,3 H TdR掺入法测cpm值 ;IL 2、IL 1 5和IL 2 +IL 1 5组诱导PBMC中NK和LAK细胞的发育 ,4h51 Cr释放实验检测对K5 6 2和LiBr细胞的杀伤活性。结果　IL 2和IL 1 5都能诱导CTLL细胞的增殖和NK、LAK细胞的杀伤活性 ,IL 2和IL 1 5可明显协同上调CTLL的增殖和LAK细胞的杀伤活性。结论　IL 2和IL 1 5在免疫调控和应答中有一定的协同作用 。Objective To explore whether IL 2 and IL 15 have synerg istic effects in the immune regulation. Methods Proliferation a ssays were performed to detect the biological activities of IL 2 and IL 15. 51 Cr release assay were employed to detect the cytotoxicity of NK and LAK cells stimulated by IL 2,IL 15 or IL 2 plus IL 15. Results IL 2 and IL 15 could promote the proliferation of CTLL and induce the cytotoxicity o f NK cells and LAK cells. The costimulatory proliferation of CTLL and cytotoxici ty of human LAK cells were markedly synergized by IL 2 plus IL 15. Con clusion The synergistic effects of IL 2 and IL 15 provide an experime ntal basis for clinical treatment of tumor.

关 键 词：IL-2 IL-15 协同调节 T细胞 增殖 LAK细胞 杀伤活性 

分 类 号：R392.11[医药卫生—免疫学]