红豆杉细胞培养产物提取策略及其对紫杉醇的影响  

Influence of Taxol Production by Product Extraction Strategies in Cell Cultures of Taxus chinensis

作　　者：罗杰[1] 刘凌[1] 胡道伟[1] 

机构地区：[1]华中科技大学生命科学与技术学院,武汉430074

出　　处：《武汉植物学研究》2003年第2期165-169,共5页Journal of Wuhan Botanical Research

基　　金：国家"8 63"高技术发展计划项目 (10 2 -12 -0 6-0 1)

摘　　要：研究了硫酸铈铵及原位提取对红豆杉细胞悬浮培养过程中细胞生长、紫杉醇合成及释放的影响。红豆杉细胞悬浮培养过程中培养第 12 d添加 2 mg/ L 硫酸铈铵能获得最大紫杉醇产量 8.3mg/ L,其中 2 .4 mg/ L 释放到细胞外 ,分别为对照组的 4倍及 12倍。同时添加 2 mg/ L 硫酸铈铵、5 %油酸 (v/ v)时胞外紫杉醇产量达到 9mg/ L,为对照组的 4 5倍。将硫酸铈铵及原位提取与补料培养相结合 ,最高紫杉醇产量可达 2 4 .5 mg/ L,其中 6 0 %释放到胞外。The effects of product release and in situ extraction on cell growth and taxol release and accumulation in cell suspension cultures of Taxus chinensis were investigated. Addition of 2 mg/L ammonium cerous sulphate on day 12 gave the greatest taxol production at 8 3 mg/L, among which 2 4 mg/L was released out of the cell, which was 4 and 12 times that of the control culture. Extracellular taxol production reached 9 mg/L when 2 mg/L ammonium cerous sulphate and 5% oleic acid (v/v) were added together, which was 45 times that of the control culture. The highest taxol accumulation of 24.5 mg/L with the release ratio of 60% was obtained when 2 mg/L ammonium cerous sulphate, 5% oleic acid (v/v) and feeding solution with 20 g/L sucrose were added.

关 键 词：红豆杉 紫杉醇 硫酸铈铵 原位提取 

分 类 号：Q813.11[生物学—生物工程]