快原子轰击质谱(FAB-MS)法与抗氧化作用的相关性  

Study on the Relationship Between Fast Atom Bombardment Mass Spectrometry and Antioxidative Effect

作　　者：贺玖明[1] 再帕尔·阿不力孜[1] 羽野芳生 小川亮 刘站柱[1] 陈世智[1] 

机构地区：[1]中国医学科学院,中国协和医科大学药物研究所,北京100050 [2]日本东邦大学药学院

出　　处：《质谱学报》2003年第2期346-350,共5页Journal of Chinese Mass Spectrometry Society

摘　　要：在探讨维生素 E的抗氧化作用机制的基础上 ,结合 FAB-MS谱中 M+· 的形成过程 ,对 FAB-MS法与药物抗氧化作用的相关性进行了研究 ,利用循环伏安法测定氧化峰电位 ,并进行比较。研究表明 :FAB-MS谱中 M+· 峰的相对强度在一定程度上可以反映化合物的抗氧化作用强弱 。Based on the antioxidant mechanism of α-tocopherol and the formation process of molecular ion M +· under FAB conditions, the relationship between the FAB-MS and antioxidative effect is discussed,and it is found that they all involve a one-electron transfer. Cyclic Voltammogram (CV) is carried out to get the oxidation potentials (Epa) which can reflect the antioxidative effect of compounds. Furthermore, the ratios of I(M +·)/I( +) evaluated from the FAB-MS spectra are correlated with the Epa, the ratios are higher, the Epa are lower. Therefore, it is demonstrates that the intensity of M +· in FAB-MS spectra can reflect the antioxidative effect of compounds to some extend, FAB-MS techniques can provide a useful clue to screen the antioxidative activity of compounds.

关 键 词：快原子轰击质谱 FAB-MS法 抗氧化作用 相关性 抗肿瘤活性物质 分子离子 维生素E 药物分析 

分 类 号：R917[医药卫生—药物分析学] R962[医药卫生—药学]