小鼠2-细胞胚胎ATP合成酶6基因特异表达分析及鉴定  

Analysis and Identification of a Stage-specific ATPase 6 Gene Expression in Mouse 2-Cell Embryo

作　　者：杨立新[1] 郁卫东[1] 陈清轩[1] 

机构地区：[1]中国科学院遗传与发育生物学研究所,北京100080

出　　处：《中国生物化学与分子生物学报》2003年第2期201-204,共4页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家重大基础研究发展规划 ( 973)资助 (No .2 0 0 0 1610 7)~~

摘　　要：合子基因组活化是小鼠胚胎早期发育由细胞质调控向核调控转变的关键 .小鼠合子基因组活化发生在 2 细胞胚胎阶段 ,通过对 2 细胞胚胎阶段特异性表达基因的分析 ,可以从分子水平上揭示早期小鼠胚胎的发育机理 .用DD RTPCR技术 ,从单个小鼠 2 细胞胚胎与成熟卵母细胞 (MII细胞 )中分离了 2个差异片段 ,片段 2同小鼠睾丸中表达的一个未知片段具有高度同源性 .经过cDNA文库构建、筛选 ,分离到其全长cDNA .序列分析结果表明 ,该基因为小鼠ATP合成酶亚单位 6基因 .ATP合成酶亚单位 6基因由线粒体DNA编码 ,与细胞内ATP的合成相关 .小鼠 2 细胞胚胎特异表达的ATP合成酶亚单位Zygotic gene activation (ZGA) is the critical event that governs the transition from maternal to embryonic control of development. In the mouse, ZGA occurs during the 2-cell stage and appears to be regulated by the time following fertilization. Development is driven by the action of a series of stage specific expressed genes. Gene expression in 2-cell mouse embryo and MII-cell was examined with mRNA differential display. The sequence analysis indicated that the gene is coincident with the ATPase 6 gene,which is encoded by mitochondrial DNA and is essential for the production of ATP. This result indicates that the expression of ATP synthesis related genes at the 2-cell stage may be required to maintain normal development.

关 键 词：小鼠 胚胎发育 早期发育 ATP合成酶亚单位6基因 DD-RTPCR技术 合子基因组活化 2-细胞胚胎 特异表达 鉴定 

分 类 号：Q954.4[生物学—动物学] Q786