异源三倍体黄瓜的离体繁殖和鉴定  

Micropropagation and Identification of an Allotriploid Cucumber in vitro

作　　者：陈劲枫[1] 罗向东[1] 余纪柱[2] 钱春桃[1] 庄飞云[1] 任刚[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室,南京农业大学蔬菜研究所,南京210095 [2]上海市农业科学院园艺研究所,上海201106

出　　处：《植物生理学通讯》2003年第2期109-112,共4页Plant Physiology Communications

基　　金：国家高技术研究发展计划专项经费 ( 2 0 0 1AA2 4112 3 ;2 0 0 2AA2 412 5 1;2 0 0 2AA2 0 70 12 );国家自然科学基金( 3 0 170 64 4);教育部<跨世纪优秀人才培养计划>;教育部科技研究重点项目 ( 0 10 97);"留学回国人员科研启动基金"

摘　　要：对甜瓜属双二倍体种 (CucumishytivusChenandKirkbride,2n =4x =38)与栽培黄瓜“北京截头”(C .sativuscv .Bei jingjietou,2n =2x =14 )杂交的种子进行胚拯救。假定为杂种植株的顶芽和腋芽用于离体繁殖的结果表明 :在MS +2 .2mg·L-16 BA和MS +3.0mg·L-1KT +0 .2mg·L-1NAA培养基上诱导不定芽的增殖系数分别为 6 .5 3和 6 .6 3;丛生芽在MS +0 .2mg·L-16 BA的培养基上伸长 ,大约 10d后取整齐一致的芽用于生根 ,在 1/ 2MS +0 .2mg·L-1IBA上生根率达91.7%。田间组培苗的单性结实能力比“双亲”更强 ;果刺黑色 ,与C .hytivus相同 ;叶深绿色 ,不同于双二倍体特有的淡黄绿色而与“北京截头”一致 ;花粉粒可染率不到 10 % ,根尖体细胞染色体鉴定为为 2n =3x =2 6 ,从而确定其为异源三倍体黄瓜。Apical and axillary buds of putative allotriploid cucumber through embryo rescuing, and from a cross between the amphidiploid species ( Cucumis hytivus Chen and Kirkbride, 2 n=4x =38) and cultivated cucumber Beijingjietou ( C. sativus , 2 n=2x =14) were used as explants to establish the micropropagation system. The best media for the induction of adventitious buds were MS+2.2 mg·L -1 6 BA and MS+30 mg·L -1 KT+0.2 mg·L -1 NAA, on which the proliferation coefficient were 6.53 and 6.63 respectively. Adventitious buds on the medium MS+0.2 mg·L -1 6 BA extended. 91.7% shoots rooted on 1/2MS +0.2 mg·L -1 IBA in 10 d. Compared to the parents, the planlets have following traits: more powerful parthenocarpy, black thorn as in C.hytivus ; dark green leaves are different from pistachio leaves of C.hytivus but 'Beijingjietou'. The stainability of pollen grains was below 10%. The chromosome number of plantlets is revealed to be 26 by cytogenetic analysis. So it was conformed to be allotriploid cucumber.

关 键 词：黄瓜 异源三倍体 离体繁殖 鉴定 远缘杂交 品种培育 

分 类 号：S642.2[农业科学—蔬菜学]