川牛膝多糖的分离、纯化及单糖组成  

ISOLATION, PURIFICATION AND MONOSACCHARIDE COMPOSITION OF POLYSACCHARIDE FROM CYATHULA OFFICINALIS KUAN

作　　者：刘颖华[1] 何开泽[1] 张军峰[1] 蒙义文[1] 

机构地区：[1]中国科学院成都生物研究所,成都610041

出　　处：《应用与环境生物学报》2003年第2期141-145,共5页Chinese Journal of Applied and Environmental Biology

基　　金：中国科学院知识创新工程项目;中国科学院成都地奥科学基金(DASF)资助项目~~

摘　　要：采用水提醇沉法提取川牛膝多糖粗品GPC,经685弱碱阴离子交换柱层析和Bio-Gel P2凝胶柱层析进一步纯化,得到川牛膝多糖RCP(refined Cyathula officinalis Kuan polysaccharide).紫外扫描、高效液相色谱、聚丙烯酰胺盘状凝胶电泳证明RCP为均一组分.质谱图显示RCP的分子量(Mr)主要分布在1000～2200.薄层正交试验确定了RCP完全水解的最优条件.薄层层析及高效液相色谱-蒸发光散射检测(HPLC-ELSD)技术揭示,RCP单糖组成为D-果糖和D-葡萄糖.Polysaccharide RCP was isolated and purified from Cyathula officinalis Kuan by hot water extraction, alcohol precipitation , 685 alkalescent anion exchange resin and Bio-Gel P2 polyacrylamide gel chromatography. Its homogeneity was proved by ultraviolet scanning, polyacrylamide disc electrophoresis and HPLC. Mass spectrum of RCP showed its molecular weight ranged mainly from 1 000 to 2 200. The optimal complete hydrolysis condition of RCP was established by TLC-orthogo-nal design. Its monosaccharide composition was determined by TLC and HPLC-ELSD, indicating RCP consisted of D-fructose and D-glucose. Fig 11, Tab 2, Ref 18

关 键 词：川牛膝多糖 分离 纯化 单糖组成 药材 水提醇沉法 

分 类 号：R284[医药卫生—中药学]