利用中国秋大豆(Glycine max(L.) Merr)筛选SSR核心位点的研究  

Selection of Core SSR Loci by Using Chinese Autumn Soybean

作　　者：谢华[1] 常汝镇[1] 曹永生[1] 张明辉[2] 冯忠孚[2] 邱丽娟[1] 

机构地区：[1]中国农业科学院作物品种资源研究所/农业部作物品种资源与生物技术重点开放实验室,北京100081 [2]东北农业大学生命科学学院,哈尔滨150030

出　　处：《中国农业科学》2003年第4期360-366,共7页Scientia Agricultura Sinica

基　　金：国家"973"项目大豆核心种质构建课题资助项目 (G19880 10 2 0 3 )

摘　　要：选择中国秋大豆为试验材料 ,对基因组DNA进行SSR标记筛选和鉴定。经过 2 0 0个位点在琼脂糖胶上初筛和 96个位点在变性聚丙烯酰胺胶上复鉴 ,选出 6 0个位点 ,这些位点具有以下特点 :(1)分布在大豆 2 0个整合遗传连锁群 ,相邻位点间平均遗传距离在 5 0cM左右。除连锁群C2 、O上分别有 5个位点 ,G、K、M上分别有 2个位点外 ,其余 15个连锁群均分布有 3个位点 ;(2 )与 96个位点在 80份秋大豆种质检测到种质间遗传关系达到极显著相关 (r =0 .910 ) ;(3)在 80份秋大豆初选核心种质中表现出较高多态性 ,平均每个位点等位变异数为 9.3,多态性信息含量 (PIC)值为 0 .773;(4)在检测的秋大豆绝大多数种质基因组中 ,均为单一拷贝的位点 ,具有较高特异性 ;(5 )在相同的PCR扩增条件下 ,同一位点不同等位变异间易于识别且扩增强度较为一致。这套SSR核心位点的确定为中国大豆核心种质的构建奠定了基础。In this study Chinese autumn soybean were used as materials, and their genomic DNAs were analyzed with SSR markers. The objective was to obtain a set of representative SSR loci for analysis of genetic diversity and DNA fingerprinting in Chinese soybean germplasm. Therefore, it will be useful for enhancing management and utilization of Chinese soybean germplasm resources. Ninety six loci from 200 loci were screened out by resolving alleles with agarose gels, and then detected alleles with silver stained polyarylamide gels. A set of 60 representative SSR loci was obtained which had following characteristics:(1) they distributed on 20 integrated linkage groups with an average of 50 cM genetic distances between two adjacent loci,including 5 loci on each of C 2 and O, 2 loci on each of G,K and M,and 3 loci on each of the other 15 linkage groups;(2) Defined the relationship among Chinese autumn soybeans were greatly significant correlation with that using 96 loci(r= 0.910 *);(3)They showed a higher level of polymorphism with an average of 8.8 alleles and 0.773 value of polymorphism informative content (PIC) per locus detected in the 80 autumn soybeans;(4) Each of them had single loci in majority of the 80 autumn soybeans and exhibited specificity;(5)Different alleles per locus were easy to discriminate and revealed approximately uniform amplified intensity under the same PCR conditions. This set of core SSR loci will play a very important role in establishment of Chinese soybean core collection.

关 键 词：SSR标记 SSR核心位点 大豆 遗传多样性 指纹图谱 核心种质 筛选 鉴定 

分 类 号：S565.1[农业科学—作物学]