AFLP初析小豆栽培和野生变种(Vigna angularis var.angularis and var.nipponensis)间演化与地理分布关系  

Preliminary Study on Geographical Distribution and Evolutionary Relationships Between Cultivated and Wild Adzuki Bean (Vigna angularis var. angularis and var. nipponensis) by AFLP Analysis

作　　者：宗绪晓[1] Duancan Vaughan Norihiko Tomooka Akito Kaga 王新望 关建平[1] 王述民[1] 

机构地区：[1]中国农业科学院作物品种资源研究所,北京100081 [2]日本农业生物资源研究所,日本茨城3058602

出　　处：《中国农业科学》2003年第4期367-374,共8页Scientia Agricultura Sinica

基　　金：IPGRI与JIRCAS联合资助的中;日;韩;朝国际合作项目 :东亚小豆 (Vignaangularis)综合种群遗传多样性分析 (1999~ 2 0 0 2 )

摘　　要：利用 12对AFLP引物 ,以饭豆标准品系M 0 0作对照 ,对来自中国、日本、韩国、尼泊尔、印度、不丹的 14 6份小豆栽培种 (Vignaangularisvar.angularis)和野生种 (Vignaangularisvar.nipponensis)种质的基因组DNA进行扩增 ,得到 313条多态性带。据AFLP多态性数据绘制的聚类图 ,可区分其中的 14 3份种质 ,表明小豆物种 (Vi gnaangularis)存在足够的遗传多样性 ,可用于资源材料的准确鉴别与分类。鉴于此 ,采用新开发的利用AFLP数据揭示核苷多样性的Innan’s进化树分析方法 ,进一步将 14 6份小豆资源分成 7个明显不同的地理演化群 ,即中国栽培种、日本栽培种、日本综合群 韩国栽培种、中国台湾野生种、中国野生种、尼泊尔 不丹栽培种和喜马拉雅野生种演化群。就上述地理演化群的遗传多样性、地理分布以及野生种与栽培种之间可能的演化关系进行了分析 ,初步认为栽培小豆至少应当有 4个不同类型的野生祖先和 3个不同的地理起源。A representative set of 146 adzuki (Vigna angularis var. angularis and var. nipponensis) germplasm from 6 Asian countries traditionally for adzuki bean production, together with an out group standard rice bean (Vigna umbellata), were analyzed by AFLP methodology using 12 informative primer pairs. 313 unambiguous polymorphic bands were created. According to the dendrogram by cluster analysis based on AFLP banding, 143 of the accessions were distinct and revealed enough genetic diversity for identification and classification of accessions within Vigna angularis. A neighbor joining tree was generated using newly developed Innan's nucleotide diversity estimate from the AFLP data; From analysis, 7 distinct evolutionary groups, named Chinese cultivated, Japanese cultivated, Japanese complex Korean cultivated, China Taiwan wild, Chinese wild, Nepal Bhutan cultivated and Himalayan wild, were detected. Nucleotide diversity with geographical distribution of each group is discussed, regarding the evolutionary relationships between wild and cultivated adzuki beans. The preliminary results indicated that cultivated adzuki bean should be domesticated from at least 4 progenitors in at least 3 geographical origins.

关 键 词：小豆 栽培种 野生变种 演化 地理分布 AFLP 遗传多样性 地理起源 

分 类 号：S521[农业科学—作物学]