雨生红球藻对紫外辐射的生理适应及超微结构变化  

Physiological adaptation and ultrastructure change of Haematococcus pluvialis exposed to ultraviolet radiation

作　　者：蒋霞敏[1] 翟兴文[1] 王丽[2] 陆开形[1] 

机构地区：[1]宁波大学生命科学与生物工程学院,浙江宁波315211 [2]浙江大学医学院,浙江杭州310031

出　　处：《水产学报》2003年第2期105-112,共8页Journal of Fisheries of China

基　　金：浙江省自然基金资助项目(301208)

摘　　要：用不同剂量的紫外线辐射雨生红球藻,测定了生长K值、色素含量、SOD、POD、CAT酶活性的变化,同时用TEM观察了细胞超微结构的变化。结果表明,紫外辐射对雨生红球藻有致死效应,致死率随着剂量升高而加大,辐射8min后藻细胞全部致死。辐射时间小于4min的存活抗性藻株生长率明显高于对照组;紫外辐射后细胞叶绿素和类胡萝卜素含量增加,增幅随着辐射时间的延长而加大。紫外辐射4～5min能使红球藻细胞虾青素含量显著提高;紫外辐射1～4min,细胞中SOD活性随着剂量增大极显著升高,但CAT酶活性降低(不显著),紫外辐射5min时SOD、CAT都失活,叶绿体、线粒体、高尔基体等细胞器都受到严重损伤。POD酶活性在紫外辐射3～5min大幅度提高。Some changes of growth rate, pigment content,SOD activity, POD activity,CAT activity and ultrastructure of Haematococcus pluvialis exposed to different dosage ultraviolet radiation have been studied,the results showed that only little ultraviolet radiation is lethal for H.pluvialis, and more microalga be died with longer illumination time till all microalga died after 8 minutes exposure,while the survivors exposed shorter than 4 minutes have higher growth rate than the control group. There is more chlorophyll and carotenoid content in the cell with longer radiation time, and remarkable increase of astaxanthin content after being radiated between 4-5 minutes has also been discovered.Contrary to the decrease of CAT activity, SOD activity has much increased to resist the ultraviolet radiation till both losing activity after 5 minutes exposure when some of their organelles were damaged inreversibly by ultraviolet radiation,such as chloroplast, plastosome and Golgi body, and POD activity has also much increased after 3-5 minutes exposure.

关 键 词：雨生红球藻 紫外线辐射 超微结构 致死效应 致死率 剂量 辐射时间 抗氧化酶 色素含量 

分 类 号：Q949.212[生物学—植物学]