低剂量铅对原代培养的海马神经元的影响  

Impact of low-level lead on growth and survival of primary cultured hippocampus neurons

作　　者：胡前胜[1] 董胜璋[1] 陈学敏[2] 傅洪军[1] 任铁玲[1] 

机构地区：[1]中山大学公共卫生学院,广州510080 [2]华中科技大学同济医学院公共卫生学院

出　　处：《中国公共卫生》2003年第5期518-520,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金资助 (30 2 71 1 0 9)

摘　　要：目的　研究低剂量铅对原代培养的海马神经元生长及存活的影响。方法　建立新生大鼠海马神经元原代无血清培养技术 ,通过不同浓度PbCl2 染毒后 ,于不同时间点观察神经细胞的生长和存活情况。结果　极低浓度的铅 ( 10 -7mol/L)即可引起神经元出芽延迟 ,突起长度缩短 ,细胞存活率下降 ,随剂量增加和时间延长 ,毒效应越严重。结论　低剂量铅具有抑制神经元生长和存活的毒性。Objective To study the impact of low-level lead on the growth and survival of primary cultured hippocampus neurons.Methods To establish the technique of primary culturing hippocampus neurons of newborn rat with serum-free medium.Being treated with different dosage of PbCl 2,the growth and survival of the cultured cells were observed at different time-point.Results Even low level of 10 -7 mol/L Pb 2+ could delay the neurite's outgrowth,shorten the neurite's length and decrease the neuron's survival rate.When Pb 2+ increased,its toxic effects increased.Conclusion Low-level lead inhibited the neurons' growth and survival.

关 键 词：铅 海马 神经元 神经突起 

分 类 号：R991[医药卫生—毒理学] R995[医药卫生—药学]