硫芥诱导淋巴细胞凋亡与细胞周期的关系  

Relationship between apoptosis and cell cycle of lymphocytes induced by sulfur mustard

作　　者：赛燕[1,2] 陈志龙[1,2] 刘勇[1,2] 赵吉清[1,2] 吴强[1,2] 王仕丽[1,2] 董兆君[1,2] 

机构地区：[1]第三军医大学预防医学系毒理学教研室,重庆410038 [2]第二军医大学海医系防化教研室

出　　处：《中国公共卫生》2003年第5期521-522,共2页Chinese Journal of Public Health

基　　金：全军"十五"重点课题 (0 1L0 54)

摘　　要：目的　探讨硫芥诱导体外培养大鼠脾脏淋巴细胞凋亡及与细胞周期的关系。方法　体外分离培养大鼠脾脏淋巴细胞。以流式细胞仪、琼脂糖凝胶电泳分别检测细胞凋亡 ,细胞周期 ,和DNA片段改变。结果　硫芥可诱导淋巴细胞凋亡 (P <0 0 5 ) ,对细胞周期有明显影响 ,主要是S期和G2 /M期明显降低 ,引起淋巴细胞生长阻滞在G1期 ;流式细胞仪PI染色出现亚二倍体峰 (凋亡峰 ) ,其G1期DNA含量明显增加 ,S期含量减少 ;琼脂糖凝胶电泳呈典型的“梯状”带 (DNAladder)。结论　硫芥可抑制体外培养的淋巴细胞由G1期进入S期 ,抑制体外培养的淋巴细胞增殖 ,通过诱导淋巴细胞凋亡实现作用机制。Objective To study the relationship between apoptosis and cell cycle of spleen lymphocyte treated with sulfur mustard.Methods Lymphocytes were separated from the spleen of Wistar rats and were cultured in vitro.Using flowcytometry ,DNA agarose gel electrophoresis,apoptosis,cell cycle,DNA fragments were detected respectively.Results Sulfur mustard could induce apoptosis on lymphocyte.And also,sulfur mustard could affect cell cycle obviously,mainly causing S and G2/M cycle decreased and lymphocyte blocked at G1 cycle stage.The DNA content of lymphocyte increased in G1 cycle,and decreased in S cycle.Hypodiploid DNA peak (apoptosis spike) was detected by FCM assay.The DNA agarose gel electrophoresis showed a ladder strap (DNA ladder).Conclusion Lymphocyte could be arrested at G1/S cycle stage and the growth of cell cycle could be suppressed by sulfur mustard.The mechanism might be carried out through sulfur mustard-induced apoptosis in lymphocyte.

关 键 词：硫芥 淋巴细胞 凋亡 细胞周期 

分 类 号：R994.7[医药卫生—毒理学]