培养软骨、关节软骨、生长板和半月板的超微结构研究  

ULTRASTRUCTURE OF CULTURED CARTILAGE, ARTICULAR CARTILAGE,GROWTH PLATE AND MENISCUS

作　　者：王建[1] 杨志明[1] 秦廷武[1] 解慧琪[1] 

机构地区：[1]四川大学华西医院骨科

出　　处：《中国修复重建外科杂志》2003年第3期247-250,共4页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家重点基础研究发展规划项目(973);单一细胞组织工程研究基金资助项目 (G1 9990 5430 8)~~

摘　　要：目的　探讨离心管培养软骨作为移植材料的可能性。　方法　 3周龄兔关节软骨细胞经离心管培养 2周形成软骨 ,另取 6周龄兔肱骨头关节软骨、胫骨近端生长板和半月板 ,并对 4种软骨进行透射电镜观察 ,比较其软骨细胞和细胞外基质结构。　结果　离心管培养软骨具有独特的超微结构 ,与关节软骨和生长板有一定的相似性 ,而与半月板有明显区别。 4种软骨都形成了各自不同的细胞和细胞外基质特征 ,离心管培养软骨呈现典型软骨细胞凋亡 ,生长板表现“黑暗”软骨细胞 ,关节软骨和半月板未见细胞凋亡。　结论　离心管培养软骨具有独特的超微结构 ,可作为关节软骨和生长板的移植物。Objective To investigate possibility of cartilage cultured in centrifuge tube as graft materials. Methods Articular chondrocytes isolated from a 3-week-old rabbit formed cartilage after cultivation for 2 weeks. Articular cartilage of humeral head, growth plate of proximal tibia and meniscus were collected from a 6-week-old rabbit. The ultrastructure of chondrocytes and extracellular matrix in the three kinds of cartilages and cultured cartilage were observed by transmission electronic microscopy. Results Cartilage cultured in centrifuge tube possessed unique ultrastructure and was similar to articular cartilage and growth plate, but it was markedly different from meniscus. The four kinds of cartilages were characteristic of respectively different chondrocytes and extracellular matrix. Cultured cartilage showed typical apoptosis of chondrocytes and 'dark chondrocytes' appeared in growth plate. Chondrocyte apoptosis was not seen in articular cartilage and meniscus. Conclusion Cartilage cultured in centrifuge tube has unique ultrastructure and may be used as graft materials for articular cartilage and growth plate.

关 键 词：培养软骨 关节软骨 生长板 半月板 超微结构 研究 组织工程软骨 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]