白介素-1β转换酶对退变腰椎间盘组织中细胞凋亡的调节  

Interleukin-1βconverting enzyme regulating apopt osis of degenerative lumbar disc tissue

作　　者：李小川[1] 赵军[2] 李力川 赵政军[1] 郭大伟[1] 

机构地区：[1]沈阳市第四人民医院骨科,辽宁省沈阳市110031 [2]沈阳医学院附属中心医院普外科,辽宁省沈阳市110024

出　　处：《中国临床康复》2003年第8期1234-1235,共2页Chinese Journal of Clinical Rehabilitation

基　　金：辽宁省自然科学基金(972270)~~

摘　　要：目的观察白介素-1β转换酶(ICE)在退变腰椎间盘组织中的表达,探讨与细胞凋亡的关系。方法应用免疫组化及流式细胞术测定50例退变腰椎间盘组织和10例正常腰椎间盘组织ICE的表达及细胞凋亡率。结果腰椎间盘组织中ICE的表达部位在软骨细胞的胞浆,退变腰椎间盘组织表达阳性率为90%,高于正常腰椎间盘组织的30%,P<0.01;阳性细胞含量为(78±13)%,高于正常腰椎间盘组织的(25±10)%,P<0.01;相对蛋白含量(FI值)为1.63±0.18,高于正常腰椎间盘组织的0.56±0.09,P<0.01;退变及正常腰椎间盘组织细胞凋亡率为(71±10)%,(20±10)%;ICE表达的FI值与腰椎间盘组织细胞凋亡率呈正相关(r=0.527,P<0.05)。结论ICE参与了腰椎间盘组织中细胞凋亡的调节,并在腰椎间盘退行性变发生和发展中有一定作用。Aim To explore the correlation between I CE and apoptosis in degenerative lumbar intervertebal disc tissue.Methods We detected theapoptotic rate and the expression of ICE in 500cases of degenerative lumb ar intervertebal disc tissues and 10ca ses of normal lumbar intervertebal d isc tissues with flow-cytometry and cyt oimmunofluoresense and immunohisto-chemistry methods.Results ICE protein lay in the cell cytoplasm of chon-drocytes both in degenerative and normal lumbar intervertebal disc tiss ues;The positive rate of ICE was 90%in deg enerative lumbar intervertebal dis c and 30%in normal tissue(P<0.01);The positive cell rate was(78±13)%in degenerative lumbar interverte bal disc and(25±10)%normal tissue(P<0.01).FI was 1.63±0.18in degenerative lu mbar interver-tebal disc and 0.56±0.09in normal ti ssue(P<0.01).The apoptotic rate was(71±10)%and(20±10)%in normal tissue.The apoptotic rate was positively correlated with ICE expr ession.Conclusion ICE upregulated apoptosis in degenerative lumbar in tervertebal disc tissue and take part in the occurrence and progression of lumbar intervertebal disc degeneration.

关 键 词：白介素-1 β转换酶 腰椎间盘退变 细胞凋亡 调节 免疫组织化学 蛋白酶类 腰椎间盘突出症 

分 类 号：Q55[生物学—生物化学] R681.53[医药卫生—骨科学]