SZZ-Harpin融合蛋白在苏云金芽孢杆菌中的表达及活性测定  

EXPRESSION AND BIOLOGICAL ASSAY OF SZZ-HARPIN FUSION PROTEIN IN BACILLUS THURINGIENSIS

作　　者：余榕捷[1] 洪岸[1] 庞义[2] 余健秀[2] 董春[3] 

机构地区：[1]暨南大学生物工程研究所,广州510632 [2]中山大学生物防治国家重点实验室,广州510630 [3]华南农业大学农学院,广州510632

出　　处：《微生物学通报》2003年第2期11-15,共5页Microbiology China

基　　金：国家自然科学基金资助项目 (No 3 9770 5 0 9)~~;国家"8 63"中试项目 (No.Z1 8 0 3 2 9)

摘　　要：PCR扩增编码SZZ短肽与植物过敏素 (harpin)融合蛋白的 1 5kb基因片段 ,克隆到苏云金杆菌 (Bacillusthuringiensis ,Bt)表达载体pHZB1上 ,并置于晶体蛋白cry1类基因的启动子下游 ,从而构建表达质粒pHSZH。将表达载体pHSZH电激转化晶体缺陷型的BtK CryB菌株 ,获得工程菌Bt pHSZH。SDS PAGE蛋白分析和生物测定结果显示 ,培养 4 8h的Bt pH SZH明显表达SZZ Harpin融合蛋白 。A 1 5 kb gene which encodes the SZZ Harpin fusion protein was cloned into the expression vector pHZB1 of Bacillus thuringiensis and located on the downstream of cry1 promoter The recombinant expression plasmid pHSZH was then transferred into BtK CryB strain by electroporation, and an engineered Bt strain Bt pHSZH was obtained SDS PAGE indicated that the SZZ Harpin fusion protein was obviously expressed in Bt pHSZH cultured for 48 hours The results of bioassay showed that the fusion protein had the ability of eliciting hypersensitive response (HR) And the strain Bt pHSZH was able to trigger the systemic acquired resistance (SAR) in tobacco

关 键 词：植物过敏素 过敏反应 系统性获得抗性 SZZ-Harpin融合蛋白 苏云金芽孢杆菌 

分 类 号：Q786[生物学—分子生物学] S432.2[农业科学—植物病理学]