心肌梗死临床诊断中血清肌酸激酶B亚基含量的动态变化  

Detection of Dynamic Changes an Fi-CK-B for Acute Myocardial Infarction in Clinic Diagnosis

作　　者：张燕燕[1] 邓汉华[2] 谢斌 

机构地区：[1]湖北药检高等专科学校临床医学教研室,邮政编码武汉430064 [2]武汉大学人民医院心内科 [3]湖北省黄州市第一人民医院

出　　处：《微循环学杂志》2003年第2期18-19,共2页Chinese Journal of Microcirculation

摘　　要：目的 :为提高心肌梗死的早期诊断水平 ,本文探讨用脑肌酸激酶单克隆抗体酶联免疫吸附法对血清肌酸激酶B亚基含量进行测定。方法 :用BCK McAbELISA法测定B亚基含量 ,动力学法测定CK、CK MB的活性 ,并对结果进行动态观测及比较。结果 :血清CK B亚基含量在心肌梗死急性发作期为 344 .89± 2 0 5 .18ng/ 5 0 μl;正常对照组和缓解期B亚基分别为 6 .5× 10 - 1 ± 2 .2× 10 - 4 ng/ 5 0 μl和 18.5 0× 10 - 4 ± 12 .2 0× 10 - 4 ng/ 5 0 μl。血清B亚基在心肌梗死急性发作期、缓解期、对照组中含量变化有显著性差异 (P <0 .0 5 )。结论 :BCK McAbELISA法可检测血清中微量B亚基。当心肌梗死急性发作 ,CK、CK MB尚在正常范围时 ,B亚基含量已明显升高。使用本法测定血清B亚基含量具有准确、快速、灵敏、可靠、特异性高等特点 。Objective: In order to improve the clinical diagnosis of Acute myocardial infarction,in this paper investigates the subunit of CK McAb ELISA with CK B to test the content of CK B.Method: Detected the concentration of CK B subunit by ELISA and to assay activity of CK MB by the onethod of dynamies.Results: Both the acute period in AMI was 344.89±205.18 ng/50 /μl and the relieved period was 18.50×10 -4 ±12.20×10 -4 ng/50 μl,that were are higher than the nomat control group (6.5×10 -1 ±2.2×10 -4 ng/50 μl) it was significant differences( P<0.05 ).Conclusion: This method for Bck McAb ELISA is an effective and predise method for detection of serum CK B during AMI in acute period also CK,CK MB delayed in a normol rauge,that CK B had been increased obviously,this method is a good way for clinical diagnose on AMI.

关 键 词：心肌梗死 临床诊断 血清 肌酸激酶 B亚基 含量 肌酸激酶同功酶 

分 类 号：R542.22[医药卫生—心血管疾病]