用3种方法检测Q流域地面水致突变活性  

Detection of Mutagenicity of Q River Surface Water by Three Methods

作　　者：吴南翔[1] 何继亮[1] 杨寅楣[2] 金锋[2] 金力奋[1] 

机构地区：[1]浙江大学医学院劳动卫生与环境卫生研究所,浙江杭州310006 [2]浙江省医学科学院卫生学研究所

出　　处：《浙江预防医学》2003年第5期3-5,共3页Zhejiang Journal of Preventive Medicine

摘　　要：目的 为了调查Q流域地面水致突变性。方法 用Ames试验、胞质阻断微核试验(CBMN)及彗星试验(comet assay)检验Q流域地面水14个地面水样品的致突变作用。结果 Ames试验结果表明9个样品对TA98(±S9)有致突变作用。4个样品对 TA100(-S9)有致突变作用,2个样品对TA100(+S9)有致突变作用。选择5个对TA98有致突变作用的样品及1个无致突变作用的样品,用胞质阻断微核试验及彗星试验进行检测,结果表明5个典型的对TA98有致突变作用的样品有5个可引起微核细胞率增加和细胞DNA迁移增高。而无致突变作用的样品后2项试验均为阴性。在3项短期试验中,Ames试验比另2个试验更为灵敏。结论 3个不同遗传终点的诱变试验均发现Q流域地面水部分样品具有致突变性。Objective To investigate the mutagenicity of Q river surface water. Methods The mutagenicity of 14 samples from Q river surface water were detected by the method of Ames test, cytokinesis-block micronucleus test and comet assay. Results The Ames test showed that 9 samplesinduced mutation on TA98 ( ±S9), 4 samples induced mutation on TA100 ( - S9) , and also 2 samples induced mutation on TA100 ( + 9 ). 5 samples with strong mutagenicity on TA98 and 1 sample without mutagenicity on TA98 were selected to be detected with cytokinesis - block micronucleus test and comet assay, The results indicated that the 5 samples increased the micronucleated cell rates and DNA migration, meanwhile, the one sample without mutagenicity on TA98 was negative in cytokinesis-block micronucleus test and comet assay. Ames test was the most sensitive method among the three short-term tests. Conclusions Some samples of Q river surface water were positive in the three short - term tests at different end-point of mutagenicity detection.

关 键 词：检测 Q流域 地面水 致突变活性 微核试验 AMES试验 慧星试验 

分 类 号：R123.2[医药卫生—环境卫生学]