某市饮用水有机污染物对小鼠卵巢功能的影响  

Effects of Organic Extracts of Drinking Water in a City on the Ovary of Mice

作　　者：田怀军[1] 吴德生[2] 陶锐[3] 

机构地区：[1]第三军医大学高原军事医学系高原卫生学教研室,重庆400038 [2]四川大学公共卫生学院,四川成都610041 [3]成都市卫生防疫站,四川成都610041

出　　处：《环境与健康杂志》2003年第3期153-155,共3页Journal of Environment and Health

基　　金：成都市卫生局重大科技攻关项目(成卫1997-31)

摘　　要：目的探讨城市饮用水有机污染物对雌性小鼠生殖的影响。方法将昆明种雌性小鼠随机分为4组,每组15只,其中1组为试剂对照组,其余3组为实验组。采用固相萃取技术富集经氯化消毒的出厂水中有机污染物,以12.5、25、50L/kg染毒昆明种小鼠,每天1次,连续5d。观察卵巢的病理变化。结果染毒后10d,生长卵泡和闭锁卵泡的数量显著增多(P<0.01)。特别是Ⅱ型闭锁卵泡数目增加很快,25L/kg组已是对照组的10倍。染毒后30d,正常卵泡数目未见异常,25、50L/kg组Ⅱ型闭锁卵泡数均显著高于对照组,黄体数目随剂量增加而下降,50L/kg组小鼠动情周期延长。结论城市饮用水有机污染物可影响小鼠卵泡的发育和成熟,对雌性小鼠生殖具有潜在的危害。Objective To determine the effects of organic extracts of drinking water on female mice reproductive system.Methods The Kunming female mice were randomly divided into4groups,one as reagent control group,the other three as treated groups,15female mice per group.The treated groups were treated with the organics extracted from the chlorinated drinking water by the solid phase extraction at the doses of12.5,25and50L/kg respectively,once a day,continuously for5d.The pathological changes of ovary were observed by optical microscopy.Results At the10th day after treatment,the number of intermediate follicles andⅡatretic follicles in ovary in the treated group significantly increased,especially the number ofⅡa-tretic follicles in25L/kg group,which was10times as high as that in the control group.At the30th day after treatment,the number of normal follicles revealed no significant changes,the number ofⅡatretic follicles in the25and50L/kg group was still significantly higher than that in the control group,the number of corpora lutea in ovary significantly decreased with the in-creases of doses.The estrous cycle delayed at the level of50L/kg.Conclusion The organic extracts from drinking water had adverse effects on the female reproductive system by affecting the development and maturity of the follicles.

关 键 词：饮用水 有机污染物 生殖毒性 

分 类 号：R123.5[医药卫生—环境卫生学] R994.6[医药卫生—公共卫生与预防医学]