一种用于中药纯化过程的近红外光谱分析新方法  

An Approach to Purifying Process Analysis of Chinese Herbal Extracts Using NIRS

作　　者：杨南林[1] 程翼宇[1] 瞿海斌[1] 

机构地区：[1]浙江大学药学院,杭州310027

出　　处：《化学学报》2003年第5期742-747,共6页Acta Chimica Sinica

基　　金：国家重点基础研究发展规划 (973计划 ;No.G1 9990 5440 5)资助项目

摘　　要：针对中药大孔吸附树脂纯化过程缺乏在线检测分析手段等问题 ,提出基于光纤近红外透射光谱的过程分析新方法 .以黄连提取物为例 ,采用HPLC分析测定值作参比 ,用偏最小二乘算法建立了近红外透射光谱校正模型 ,并成功地用于预测黄连生物碱在大孔树脂纯化过程中的洗脱曲线 .本方法实时、快速 ,可同时测定洗脱物中盐酸小檗碱、盐酸巴马亭、盐酸药根碱和黄连总生物碱的浓度 ,预测精度满足工业过程分析要求 ,为提高中药质量分析水平提供了新的途径 .A new method for process analysis of the production of herbal medicine, which is based on fiber optic near infrared transmission spectroscopy (NIRTS) is developed. The purification process. of Coptis chinensis extracts with nonionic macroreticular resin was selected as an example. HPLC was used to determine the concentrations of some alkaloids for reference. Four calibration models were then developed to correlate NIRTS spectra with the concentration. values of the alkaloids determined by HPLC using PLS algorithm. The models were applied to successfully predict the elution curves of the alkaloids on macroreticular resin. The results showed that the concentrations of Jatrorrhizine, Palmatine, Berberine and total alkaloid in, elutes can be, accurately determined by NIRTS simultaneously. The new method is fast and real time, providing a new tool for quality control of Chinese herbal medicine products.

关 键 词：中药 纯化过程 近红外光谱分析 大孔吸附树脂 在线检测 偏最小二乘算法 黄连 过程分析化学 

分 类 号：O657.3[理学—分析化学]