酸碱度对对硝基酚呈色反应研究及应用  

Study and Application on Effects of Different pH on P-Nitrophenol Coloring

作　　者：李研[1] 张孝山[1] 李瑞兰[1] 潘彤[1] 

机构地区：[1]天津市血液中心,300110

出　　处：《天津医药》2003年第5期310-312,共3页Tianjin Medical Journal

摘　　要：目的 :研究不同酸碱度 (pH)对对硝基酚(PNP)呈色反应的影响。方法 :进行酸、碱性PNP吸收峰扫描 ,在奥林巴斯AU -400自动分析仪上建立检测程序 ,评价不同 pH缓冲液对不同波长下PNP吸光度变化的影响。结果 :酸性时PNP最大吸收峰为295～340nm ,碱性时PNP最大吸收峰为375～420nm ;建立精密度检测参数。结论 :根据PNP呈色特点 ,检测自动生化分析仪340～410nm精密度 ,该方法简单、经济、可操作性强。Objective:To study and apply the effects of different pH on pˉnitrophenol(PNP)coloring.Methˉods:In order to evaluate the effects of buffers with various pH on absorbent changes of PNP at different wavelength,the measuring parameter for Olympus AUˉ400was established by scanning obsorbent peak of PNP dissolved in acidic or alkaline solutions.Results:The biggest absorbent peak ranges of PNP at acidic and alkaline pH were from295nm to340nm and fron375nm to420nm respectively.The measuring parameters at340nm and410nm for precision of AUˉ400analyzer were separately set up.Conclusion:According to speciality of PNP coloring,the precisions of automatic analyzer at340nm～410nm were determined.The method was simple,eˉconmic and operable.

关 键 词：酸碱度 对硝基酚 呈色反应 比色法 AU-400自动分析仪 

分 类 号：R446[医药卫生—诊断学]