腹泻患者及培养基中人芽囊原虫的各期形态研究  

Study on Morphology of Blastocystis hominis in Culture and from Diarrhea Patients

作　　者：张旭[1] 乔继英[2] 董小惠[3] 李亚青[4] 李小琪[1] 李琛[1] 

机构地区：[1]西安交通大学医学院寄生虫学教研室,西安710061 [2]西安交通大学医学寄生虫学教研室,西安710061 [3]西安交通大学医学院微生物学教研室,西安710061 [4]西安交通大学医学院医学检验中心,西安710061

出　　处：《中国寄生虫学与寄生虫病杂志》2003年第2期116-118,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：卫生部科学研究基金(No.98-1-231)

摘　　要：目的 研究光镜下各期人芽囊原虫(Blastocystis hominis,B.h)形态,以进一步研究其生活史及致病性,并为临床检验提供形态依据。方法 使用洛克氏液-鸡蛋-血清培养基,对腹泻患者粪便的B.h进行体外连续培养,经碘液染色及铁苏木素染色,在光镜下研究其各期形态、结构。结果 通过形态学研究观察到空泡型、颗粒型、阿米巴型及包囊型以及各型之间的相互转化。结论 B.h形态多样,临床多见空泡型和颗粒型。空泡型可成囊。Objective To study morphology of different stage Blastocystis haminis (B.h) for establishing a base in the research of life cycle and pathogenicity of B. h and providing information for clinical laboratory. Methods B. h from diar-rheal patients was continuously cultured in LES medium, and morphology of B.h was studied with iodine and iron hematoxylin staining under light microscope. Results The vacuolar, granular, amoeboid and cyst forms of B. h and transformation among the forms were observed microscopically. Conclusion Among different forms, the vacuolar and granular forms were often seen clinically and the vacuolar form can transform to cysts.

关 键 词：人芽囊原虫 腹泻 粪便培养 形态学 空泡型 颗粒型 

分 类 号：R531[医药卫生—内科学] R382.9[医药卫生—临床医学]