急性排斥反应过程中淋巴细胞趋化因子基因表达的意义  

Implication of expression of lymphotactin mRNA in allografts

作　　者：顾晓 唐孝达[2] 顾沈阳 杨尚琪[2] 周佩军[2] 徐达[3] 王祥慧[3] 谭建明[2] 

机构地区：[1]扬州大学临床医学院泌尿外科,江苏省225001 [2]上海市第一人民医院上海市器官移植临床中心 [3]上海第二医科大学附属瑞金医院泌尿外科

出　　处：《中华器官移植杂志》2003年第3期160-162,共3页Chinese Journal of Organ Transplantation

基　　金：上海市医学领先专业重点学科发展基金资助项目 ( 9830 0 5)

摘　　要：目的　探讨急性排斥反应过程中淋巴细胞趋化因子 (LTN)mRNA在移植心脏局部表达的意义及环孢素A(CsA)对其的影响。方法　以SD大鼠为受者 ,Wistar大鼠为供者 ,施行异位 (腹腔 )心脏移植术 ,分为术后使用CsA组 (15mg·kg-1·d-1)和不用组 ,并设SD大鼠间的心脏移植组 (同系移植组 ) ,以正常SD大鼠为对照组。采用一步法逆转录 聚合酶链反应 (RT PCR)检测术后不同时间移植心脏局部LTNmRNA的表达。结果　正常对照组和同系移植组在各时间点均未见LTNmRNA表达 ;LTNmRNA在未用CsA组的表达变化与急性排斥反应的进程相关 ,排斥反应的早期出现LTNmRNA表达上调 ,术后 5d时达到峰值 ,而应用CsA组 ,LTNmRNA的表达峰值出现延缓 ,且明显低于未用CsA组 (P <0 .0 5 )。结论　LTNmRNA的表达上调与排斥反应过程中淋巴细胞浸润密切相关 ,可能对急性排斥反应的早期诊断有帮助 ;CsA可抑制LTN基因的表达 。Objective To investigate the implication of lymphotactin (LTN) mRNA expression in cardiac allografts and the effect of cyclosporin A (CsA).Methods Three groups of rats underwent the heterotopic cardiac transplantation: isograft group (group A), untreated group (group B) and CsA treated group (group C). The LTN mRNA expression was detected by one step RT PCR method. Results The expression of LTN mRNA was not detected in both isografts and normal hearts. The changes of LTN mRNA expression were correlated with the process of acute allograft rejection. The peak of elevated level of the LTN mRNA expression appeared in the 5th day after transplantation and CsA could delay the peak and downregulate its expression. The peak level of LTN mRNA expression in group C was significantly lower than that in group B ( P < 0.05 ).Conclusion Upregulation of LTN mRNA expression was significantly correlated with the trafficking of lymphocytes and may be useful to diagnose the acute allograft rejection earlier. It may be one of the important mechanisms of CsA to intervene the acute rejection by downregulating the LTN mRNA expression.

关 键 词：异位心脏移植术 急性排斥反应 淋巴细胞趋化因子 基因表达 环孢素A 

分 类 号：R654.2[医药卫生—外科学] R392.4[医药卫生—临床医学]