黄芪对TGF-β1致人腹膜间皮细胞分泌细胞外基质的影响  

Influence of huangqi on TGF-beta 1 inducing extracellular matrix secretion of human peritoneal mesothelial cell

作　　者：刘映红[1] 刘伏友[1] 段绍斌[1] 肖平[1] 袁芳[1] 彭佑铭[1] 成枚初[1] 夏家辉[2] 

机构地区：[1]中南大学湘雅二医院肾内科,长沙410011 [2]中南大学医学遗传学国家重点实验室,长沙410078

出　　处：《湖南医科大学学报》2003年第2期141-144,共4页Bulletin of Hunan Medical University

基　　金：教育部高等学校重点实验室访问学者基金 (教技司 2 0 0 0 ;170 );湖南省自然科学基金项目 ( 0 1JJY2 0 71);湖南省卫生厅基金项目 ( 2 0 0 1- Y -39)

摘　　要：目的 :探讨黄芪对腹膜纤维化的作用及可能机制。方法 :人腹膜间皮细胞 (HPMC)作体外培养 ,在第三代 ,将细胞转板至细胞融合后 ,将其分为对照组 (F12 ) ,TGF β1组 (F12 +TGF β15ng/ml) ,TGF β1加黄芪 1组(F12加TGF β1加黄芪 ,黄芪终浓度为 10 0 μg/ml,TGF β1加黄芪 2组 (F12加TGF β1加黄芪 ,黄芪终浓度为 1mg/ml) ,分别在 2 4,48h采用ELISA法检测上清液中纤维连接蛋白 (FN)和纤溶酶原抑制剂 (PAI 1)的含量 ;FNmRNA ,PAI 1mRNA和碱性成纤维细胞生长因子 (bFGF)mRNA的表达采用半定量逆转录多聚酶链反应 (RT PCR)检测。结果 :①HPMC表达FN ,PAI 1和bFGF ;②HPMC在 5ng/mlTGF β1刺激下分泌FN及PAI 1明显的增加 ,与对照组比较差异有显著性意义 (P <0 .0 5 ) ;③HPMC在TGF β1和不同浓度的黄芪刺激后 ,在 2 4h内可使FN及PAI 1减少 ,尤其是PAI 1与TGF β1组比差异有显著性意义 (P <0 .0 5 )。④HPMC经TGF β1作用 12h后 ,FNmRNA ,PAI 1mRNA和bFGFmRNA半定量结果与对照组相比 ,其表达量分别上调 18.5 4%,8.6 %和 6 6 .9%,HPMC经 10 0μg/ml和 1mg/ml黄芪作用 12h后FN ,PAI 1和bFGFmRNA的表达与TGF β1组比较均下调 ,其结果分别为5 .3 %,1.3%,3 .8%和 5 .4%,74.3 %,2 3 .7%。结论 :TGF β1可促进HPMC合成?Objective To study the effect of huangqi on peritoneal sclerosis and its possible mechanism.Methods Isolated human peritoneal mesothelial cells (HPMC) was cultured . Cultured HPMC were treated with F12 without serum (control group), F12 with TGF-beta1(TGF-beta1 group),F12 with TGF-beta1 and huangqi 100 μg/ml (huangqi 1 group), F12 with TGF-beta1 and huangqi 1 mg/ml (huangqi 2 group).Fibronectin(FN) and plasminogen activator inhibitor-1(PAI-1)were detected by ELISA method. The expression of FN mRNA,PAI-1 mRNA and bFGF mRNA was detected by RT-PCR method. Results ①HPMC expressed FN,PAI-1,and bFGF. ②Fn and PAI-1 significantly increased compared with control with 5 ng/ml TGF-beta1stimulated at 24 hours and 48 hours(P<0.05); ③The different concentration of huangqi decreased FN and PAI-1 expression compared with TGF-beta1 group,especially 1 mg/ml huangqi at 24 hours(P<0.05). ④FN,PAI-1and bFGF mRNA expression were higher in 12hours after stimulation with TGF-beta1 compared with control.FN,PAI-1and bFGF mRNA expression were lower in 12 hours after stimulation with different concentration of huangqi (100 μg/ml and 1mg/ml) than those in the TGF-beta1 group.Conclusion TGF-beta1 promotes extracellular matrix synthesis and secretion of HPMC. Huangqi partly counteracts the synthesis of human peritoneal mesothelial cell's extracellular matrix by the stimulation of TGF-beta1.

关 键 词：黄芪 TGF-Β1 腹膜间皮细胞 分泌 细胞外基质 影响 

分 类 号：R285.5[医药卫生—中药学]