电针穴位对脑缺血-再灌注大鼠海马内MDA含量及SOD、GSH-P_X活性的影响  

Influence of electroacupuncture points on MDA content and SOD、GSH-P_X activity in ischemia-reperfusion rat's hippocampus

作　　者：柏志全[1] 蒋建伟[2] 周丽丽[1] 郑辉[1] 姚平[1] 王跃春[1] 周卓妍[1] 

机构地区：[1]暨南大学医学院生理学教研室,广东广州510632 [2]暨南大学医学院生物化学教研室,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2003年第2期38-41,共4页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：广东省中医药管理局资助项目(344180)

摘　　要：　目的:观察电针对脑缺血-再灌注损伤的防护作用及可能机制。方法:采用结扎大鼠双侧颈总动脉造成的脑缺血-再灌注损伤模型,测定电针穴位前后海马内MDA含量和SOD、GSH-PX活性。每日电针双侧"百会"、"风池"、"大钟"及"足三里"穴30min,持续7和14d,疏-密波频率:2～20Hz,强度:2 0A。结果:电针能降低缺血-再灌注组大鼠海马MDA含量、提高SOD及GSH-PX活性。结论:电针穴位能提高动物脑海马内氧化酶活性、抑制自由基生成。Aim: To study the protective effects of electroacupuncture bilateral points on cerebral ischemia-reperfusion injury and its mechanism in rats. Methods: Using animal models of cerebral ischemia-reperfusion injury, measured MDA content and SOD, GSH-PX activity in hippocampus of the rats. Electroacupuncture bilateral points of 'Baihui, Fengchi, Dazhong, Zusanli' 30 min everyday and last 7 and 14 d, frequency:2～20 Hz and intensity :2.0 A. Results: Electroacupuncture could decrease MDA content and increase SOD、GSH-PX activity in ischemic-reperfusion group. Conclusion: Electroacupuncture bilateral points have protect effects on the model of cerebral ischemia-reperfusion injury in rats. The protection mechanism may relate to its antioxidation.

关 键 词：电针 自由基 脑缺血-再灌注 丙二醛 超氧化物歧化酶 谷胱甘肽过氧化物酶 

分 类 号：R245[医药卫生—针灸推拿学]