高效液相色谱-电化学法检测尿液8羟基脱氧鸟苷水平  

Measurement of Urinary 8-Hydroxydeoxyguanosine Level with a High-Pressure Liquid Chromatography Eectrochemical Approach

作　　者：彭涛[1] 严律南[1] 刘志明[2] 葛西宏 彭民浩[2] 黎乐群[2] 玉江和义 秦雪[4] 韦忠亮 梁任祥 李家荃[2] 

机构地区：[1]四川大学华西医院普外科,成都610041 [2]广西医科大学附一院普外科,南宁530021 [3]日本产业医科大学生态科学研究所肿瘤部 [4]广西医科大学附一院检验科 [5]广西扶绥县肿瘤防治研究所,扶绥532100

出　　处：《中国普外基础与临床杂志》2003年第3期281-283,共3页Chinese Journal of Bases and Clinics In General Surgery

基　　金：广西自然科学基金 (桂科自 991 2 0 2 8) ;广西自筹经费出国留学项目 (桂政办函 2 0 0 0 - 2 72号 )

摘　　要：目的　探讨一套高效液相色谱 电化学系统检测尿 8羟基脱氧鸟苷 ( 8 OH dG)水平的方法学。方法　采用ESA 5 60 0Coularray系统检测广西扶绥县肝癌户人群 2 1例及南宁市对照人群 63例尿 8 OH dG水平。 结果　 8 OH dG峰与杂质峰分离良好 ;8 OH dG浓度在 0 .0 1～ 15ng/ml间呈良好线性 ;保留时间、标样和内对照的重复性试验的批内差异 (CV)均小于 8% ;标样 (F =0 .0 0 2 ,P =1.0 0 0 )和内对照 (F =2 .965 ,P =0 .0 82 )的重复性试验的批间差异经方差分析均无显著性意义。采用上述方法测得 84例总的尿 8 OH dG水平为 ( 5 .2 6± 0 .41)ng/mg肌酐 ,高于同类研究的检测值〔( 4 .62± 0 .0 91)ng/mg肌酐 ,n =3 2〕 ,但两地人群间尿 8 OH dG水平差异无显著性意义。 结论　这是对肝癌高发区高危人群氧化性DNA损伤的首次报告。通过谨慎设计试验和优化试验参数 ,该系统对 8 OH dG峰的分离优于以往报告 ,适用于尿 8 OH dG的高通量定量分析 ,可应用于人群氧化性DNA损伤的研究。Objective To investigate the methodology of a newly developed high pressure liquid chromatography electrochemical system in urinary 8 hydroxydeoxyguanosine (8 OH dG) quantification. Methods Quantification of urinary 8 OH dG with ESA 5600 Coularray system among 21 children from liver cancer high risk areas, Fusui country, in contrast with 63 controls from Nanning city, Guangxi province.Results The resolution, sensitivity, linearity, precision and recovery of this approach all fully meet the requirement of routine urinary 8 OH dG quantification. The mean 8 OH dG level of this population was (5.26±0.41) ng/mg creatinine, higher than previous report 〔(4.62±0.091) ng/mg creatinine〕 by similar method.Conclusion With cautious design and modification, this method is reliable and accurate in high throughput quantification of urinary 8 OH dG.

关 键 词：高效液相色谱 电化学法 检测 尿液 8羟基脱氧鸟苷 肝癌 

分 类 号：R446.12[医药卫生—诊断学]