用地高辛配基标记DNA探针进行菌落原位杂交  

Colony Hybridization with Digoxingenin Labelled DNA Probe

作　　者：蒋三亮 张思仲[1] 

机构地区：[1]华西医大医学遗传学研究室

出　　处：《华西医科大学学报》1992年第3期245-247,共3页Journal of West China University of Medical Sciences

基　　金：国家自然科学基金~~

摘　　要：用地高辛配基标记DNA探针,结合酶联免疫反应检测显示信号。建立了一种简便,快速的菌落原位杂交新方法。结果证明该方法敏感、稳定,完全可取代同位素用于细菌分析和克隆筛选。A simple, rapid method for co-lony hybridization has been developed.TheDNA probes were labelled by digoxingenin.Hybridization signal was detected by enzyme-linked immunoassay reaction. The resultsshowed that this method is sensitive andreproducible, and it can be used for colonyhybridization instead of isotopic methods.

关 键 词：地高辛配基 DNA探针 菌落原位杂交 

分 类 号：R392.2[医药卫生—免疫学]