α_2巨球蛋白抑制蕲蛇毒激活兔血小板的实验观察  

α_2megaloglobulin Inhibits the Activation of Rabbit Platelet by Chinese Agkistrodon acutus Venom

作　　者：邓承祺[1] 李素琼[1] 李光蓉[2] 乔晓蓉[3] 何才云[3] 管锦霞[4] 

机构地区：[1]华西医大内科学教研室 [2]华西医大电子显微镜实验室 [3]华西医大分析测试中心 [4]广州医学院

出　　处：《华西医科大学学报》1992年第4期416-419,共4页Journal of West China University of Medical Sciences

基　　金：四川省卫生厅科学基金

摘　　要：作者用体外实验观察α_2巨球蛋白(α_2MG)抑制中国蕲蛇毒(CAAV)对免血小板的激活作用.结果表明:100μg/mlCAAV致洗涤兔血小板聚集率最大(43.8％),聚集后上清液中释放的5-HT和PF_4的量最多(0.81μg/ml和39.08％)。洗涤兔血小板聚集前或后加入α_2MG均能显著抑制CAAV致聚作用及仰制5-HT和PF_4的释放(P<0.05和<0.001)。电镜发现在洗涤兔血小板悬液中先加α_2MG后加CAAV时,血小板无聚集,血小板超微结构与未加CAAV的对照组血小板相同。An in vitro study of α_2--me-guloglobulin(α_2MG)in the inhibition of theactivation of washed rabbit platelet by C inese Agkistrodon acutus venom(CAAV) wasreported.Results showed that CAAV inducedaggregation rate of washed rabbit plateletwas positively related to the CAAV concen-tration,and the maximal aggregation ratewas 43.8±9.9% at the concentration of 100μg/ml.Meanwhile the release of serotonin(5-HT)and platelet factor 4(PF_4) from theplatelet reached maximum at the CAAV con-centrations of 0. 81±0. 07μg/ml and 39.08±2.78%, respectively. Platelet aggregationand the release of 5--HT and PF_4 inducedby CAAV could be inhibited by the addi-tion of 0.25% α_2 MG into the platelets both1 minute before and 1 minute after theaddition of CAAV.when α_2 MG was addedto the platelets 1 minute prior to the ad-dition of CAAV,the platelet aggregationand the ultrastructural changes whichcould be induced by CAAV were notobserved,and the morphology of the rabbitplatelets was the same as that of thenormal counterparts

关 键 词：巨球蛋白 中国蕲蛇毒 血小板聚集 

分 类 号：R558[医药卫生—血液循环系统疾病]