2-对甲氧基苯基-4-苯基-1,5-苯并硫氮杂-α-(N-噁唑烷酮基)-β-内酰胺的反相高效液相色谱法测定  

Determination of 2-p-Methoxyphenyl-4-phenyl-1,5-benzothiazepines -α-oxazolidonyl-β-lactam by RP-HPLC

作　　者：朱智甲[1] 王兰芝[2] 李媛[2] 

机构地区：[1]河北师范大学实验中心,河北石家庄050016 [2]河北师范大学化学系,河北石家庄050016

出　　处：《分析测试学报》2003年第3期92-93,共2页Journal of Instrumental Analysis

基　　金：河北省自然科学基金资助项目(200154)

摘　　要：建立了一种测定2_对甲氧基苯基_4_苯基_1 ,5_苯并硫氮杂_α_(N_唑烷酮基 )_β_内酰胺(MPPBOL)的反相高效液相色谱法 ,色谱柱为HypersilODS2(4.6mm×250mm,5μm) ,流动相为CH3CN -CH2Cl2-H2O(体积比70∶0.5∶30) ,检测波长240nm ;MPPBOL在0.1～10mg范围内 ,峰面积与待测物质的质量呈良好的线性关系 ,相关系数为0.9994 ,相对标准偏差3.4 % (m=1.0mg,n=6) ,回收率在97 %～103A method for the determination of 2 _ p _methoxyphenyl _ 4 _ phenyl _ 1,5 _ benzothiazepines _ α _ oxazolidonyl _ β _ lactam(MPPBOL) has been established by reversed _ phase high performance liquid chromatography with UV detection. Chromatographic separation was carried out on a Hypersil ODS2 column(4.6 mm×250 mm, 5 μm) with an eluent consisting of CH3CN-CH2Cl2-H2O(70∶0.5∶30 by volume) as a mobile phase. A good linear relationship between the peak area and the mass of MPPBOL existed in the range of 0.1～10 mg(r=0.999 4). The recovery was in the range of 97%～103% with relative standard deviation of 3.4%(m=1.0 mg, n=6). The results showed that it was an accurate, rapid and simple method for the determination of MPPBOL.

关 键 词：2-对甲氧基苯基-4-苯基-1 5-苯并硫氮杂Zhuo-α-(N-噁唑烷酮基)-β-内酰胺 反相高效液相色谱法 测定 药理活性 

分 类 号：TQ463.5[化学工程—制药化工]