肾病病人血清PAGE和ACE比较  

Research on the PAGE Map of Normal and Abnormal Human Serum for Clinic (The Group of 5 Papers)

作　　者：沈庭希[1] 舒煦 刘荣敏[1] 吴俊辉[1] 张光芬[2] 白美媛 杨荣华[2] 范昕建[3] 冯萍[3] 汪冰[3] 庄丽芳[4] 刘先蓉[5] 

机构地区：[1]四川省肿瘤研究所 [2]成都市第六人民医院 [3]华西医科大学附一院传染科 [4]华西医大附一院检验科 [5]华西医大附一院肾病科

出　　处：《华西医学》1992年第4期405-406,共2页West China Medical Journal

基　　金：四川省卫生厅资助研究课题

摘　　要：在传统的ACE方法分析肾病病人血清时,都是计量白蛋白和β球蛋白在血清中的含量以确定肾病引起的蛋白质丢失情况和肾小球、肾小管受损的情况。我们用PAGE-CP分析肾病病人血清时发现,血清中PA4蛋白的丢失比自蛋白或β蛋白更严重,大多数病例血清在PAGE—CP中PA4的丢失直接可以观察而无需扫描计量,因此PA4和白蛋白、β蛋白一样,可作为肾病病人血清蛋白含量的一个特点,PAGE—CP可作为肾病诊断的一种有效方法。By a new kind of horizontal slab electrophoresis cell, we research on the PA GE map of normal and abnormal human serum. The gel shows color dyeing with Coomassie blue G-Pararosaniline. This method we call it PA GE-CP. The normal human serum PAGE-CP map are steady and the abnormall are absence (short of) or increase some bands, the hepisoma are short of PA4; the leukaemle and bone marrow cell hyperplasia absence of δ; the nephropasias are decrease of β and PA4; If there are red stripes In the γ, the body is in mucositis. The stripes are blue. the ls in multiplex osteoma. Because the PAGE -CP (?)s resolution are high than the acetic cellulose membrane electrophoresis(ACE). The PAGE-CP maps and the technology can be used in clinic substituting for ACE.

关 键 词：肾疾病 血清 电泳 

分 类 号：R692.04[医药卫生—泌尿科学]