骨髓基质干细胞接种于纤维蛋白凝胶的体外培养  

CULTURING RABBIT BONE MARROW STROMAL CELLS IN VITRO ON THE SURFACE OF FIBRIN GLUE

作　　者：邓展生[1] 詹新立[1] 朱峥嵘[1] 王卫国[1] 

机构地区：[1]中南大学湘雅医院骨科,长沙410008

出　　处：《中国现代医学杂志》2003年第9期14-16,共3页China Journal of Modern Medicine

基　　金：湖南省科技厅科研项目 (项目号 10 13 -3 4)资助

摘　　要：目的 :研究经分离培养的兔骨髓基质干细胞 (MSC)接种在可吸收性纤维蛋白凝胶上向成骨细胞分化 ,表型维持的影响。方法 :取兔骨髓基质干细胞于含 10 0ml/L胎牛血清的DMEM培养液中培养 ,并向成骨细胞诱导。将细胞与纤维蛋白凝胶复合 ,通过扫描电镜观察细胞附着情况 ,共聚焦显微镜观察Ⅰ型胶原的分泌 ,组织化学染色检测碱性磷酸酶 (ALP)阳性细胞 ,ALP含量测定。结果 :骨髓基质干细胞在可吸收性纤维蛋白凝胶表面附着良好并继续增殖 ,可分泌Ⅰ型胶原 ,这些细胞的ALP染色呈强阳性 ,细胞碱性磷酸酶含量增高。结论 :具有良好的可塑性、低抗原性、好的降解吸收性的纤维蛋白凝胶具有促进骨髓基质细胞表达成骨细胞表型、合成细胞外基质的功能 ,是良好的细胞载体。Objective:To investigate the effect of absorbable fibrin glue as cell culturing scaffold on the differentiation of rabbit bone marrow stromal cells (MSC) .Methods:MSC were separated and cultured from bone marrow of rabbit, MSC were co- cultured with fibrin glue in vitro and induced to differentiate towards osteoblasts. ALP was examined by histochemical staining. Cell material attachment was observed with scanning electron microscope,type I collegen secretion was studied with laser confocal microscope.Results:The cells could adhere to absorbable fibrin glue and go on proliferating and secrete type I collegen. The rate of ALP positive cells was 88.42%. Conclusion:The shaped and absorbable fibrin glue is a good bone tissue engineering scaffold.

关 键 词：骨髓基质干细胞 成骨细胞 纤维蛋白凝胶 I型胶原 碱性磷酸酶 

分 类 号：R318.08[医药卫生—生物医学工程]