HLA-B血清学纯合型标本的PCR-SSP基因分型  

PCR-SSP genotyping for the samples of HLA-B homozygotes identified by serological tests

作　　者：傅茜[1] 毕颖[1] 陈连周[1] 陈规划[1] 

机构地区：[1]中山医科大学附属第一医院外科实验室,广州510080

出　　处：《临床检验杂志》2003年第3期132-133,共2页Chinese Journal of Clinical Laboratory Science

摘　　要：目的　探讨血清学和PCR SSP 2种方法在HLA B位点分型的应用价值。方法　对血清学分型HLA B位点纯合型标本 ,用PCR SSP方法进行基因分型。结果　血清学分型为纯合型的 75例标本中 ,PCR SSP方法基因分型有 12例为杂合型。结论HLA B的PCR SSP基因分型结果准确、可靠 ,而血清学分型方法成本低 。Objective To evaluate the practical value of serological tests and PCR SSP for HLA B typing.Methods PCR SSP was used for genotyping of HLA B homozygotes identified by serological tests.Result Of 75 samples of HLA B homozygotes identified by serological tests12 were found as heterozygotes by PCR SSP genotyping.Conclusions HLA B typing by PCR SSP had proved to be accurate and reliable,while the serological tests were cheap,rapid and easy to perform.

关 键 词：HLA—B 血清学分型 纯合型标本 PCR—SSP 基因分型 

分 类 号：R392.11[医药卫生—免疫学] Q52[医药卫生—基础医学]