沥水调脂胶囊在体外对弱氧化型低密度脂蛋白诱导血管平滑肌细胞增殖的影响  

Effect of Li Shui Tiao Zhi Capsule on Proliferation of Vascular Smooth Muscle Cells Induced by Mild Oxidized Low Density Lipoprotein in Vitro

作　　者：陈杲[1] 宋剑南[1] 史国峰[1] 刘卫红[1] 陈冰[1] 

机构地区：[1]中国中医研究院基础理论研究所,北京100700

出　　处：《中国中医基础医学杂志》2003年第4期52-55,共4页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金资助 ( 3 9970 883 )

摘　　要：目的 :探讨沥水调脂胶囊治疗动脉粥样硬化的作用机理和弱氧化型低密度脂蛋白在动脉粥样硬化由痰致瘀过程中的作用。方法 :以甲醇除蛋白后的沥水调脂胶囊含药血清和弱氧化型低密度脂蛋白 (mox LDL)处理血管平滑肌细胞 (VSMC) ,用3 H TdR掺入法检测DNA合成率。结果 :低浓度的除蛋白含药血清 (5 %、10 % )在 5、10 μg ml的nox LDL处理VSMC细胞组中与对照组比较 ,差异均无显著性意义 ;高浓度除蛋白含药血清 (2 0 % )对两个不同浓度的mox LDL诱导的VSMC增殖均有明显的抑制作用 (抑制率分别为 4 0 %、4 2 % ,P <0 0 5 ) ;其作用方式与维生素E相似。结论 :沥水调脂胶囊呈浓度依赖方式抑制mox LDL诱导的VSMC增殖 ,其机制可能与该药的抗氧化作用有关 ,进一步提示氧化损伤是由痰致瘀过程中的关键环节 ,以及抗氧化可能是阻止由痰致瘀的主要手段。Objective:To study the pharmacology of Li Shui Tiao Zhi capsule and the role of Mildly Oxidized Low Density Lipoprotein(mox LDL) from the Phlegm Syndrome to Blood Stasis Syndrome in atherosclerosis. Method:serum containing medicine removed protein and mox LDL treated vascular smooth muscle cells;DNA synthesis was examined by measuring 3H TdR incorporation into the cellular DNA. Results:to compare between all group in the experiment used low concentration (5%,10%) serum containing medicine removed protein and respective control group, there was not significant difference;20% serum containing medicine removed protein significantly inhibit mox LDL induce proliferation(40%、42%, P <0 05).Conclusion: Li Shui Tiao Zhi capsule may significantly inhibit proliferation of vascular smooth muscle Cells Induced by mox LDL, which implicate that mox LDL may play a important role from the Phlegm Syndrome to Blood Stasis Syndrome in atherosclerosis.

关 键 词：沥水调脂胶囊 体外 弱氧化型低密度脂蛋白 血管平滑肌 细胞增殖 动脉粥样硬化 健脾祛痰化瘀法 兔 

分 类 号：R285.5[医药卫生—中药学] R259.414[医药卫生—中医学]