冬虫夏草及人工虫草核苷类成分的TLCS研究  

Study of nucleotides in Cordyceps and its mycelia by TLC-Scanning

作　　者：米莉莉[1] 张素文 孙家进 王智华[1] 洪筱坤[1] 

机构地区：[1]上海中医药大学药学院,上海200032 [2]上海第六制药厂,上海200000

出　　处：《中成药》2003年第5期402-405,共4页Chinese Traditional Patent Medicine

摘　　要：目的 :以腺苷、尿苷、鸟苷为指标测定天然虫草与人工虫草菌丝体中核苷类有效成分的含量 ,并对不同虫草样品进行薄层色谱指纹图谱对比研究 ,探求虫草中核苷类化合物鉴别的专属性。方法 :采用高效薄层色谱扫描法进行含量测定 ,同时建立了不同虫草样品的荧光淬灭薄层色谱指纹图谱。结果 :虫草菌丝体中测得腺苷、尿苷鸟苷成分高于天然虫草。结论 :该法快速简便 ,重现性好 ,能有效鉴别出虫草中核苷类成分以及评价不同虫草样品的质量。Objective: To establish the quality assessment of Cordyceps and its cultured Cmycelia by TLC fingerprint, and to compare the content of the nucleotides between them. Methods: Adenosine, Uridine and Guannosine were detemined by TLC-Scanning samples were perfomed by fluorescence quenching analyis. Results: The contents of adenosiue, uridine, and guanosine from cordyceps mycelia were more than that of cordyceps from wildlife. Conclusion: This method is convenient and rapid, and it can effectively evaluate nucleotides in nature Cordyceps and culture Cordyceps.

关 键 词：天然虫草 人工虫草菌丝体 TLCS 腺苷 尿苷 鸟苷 

分 类 号：R284.1[医药卫生—中药学]