温室栽培光强和光质对高山红景天生物量和红景天甙含量的影响  

Effects of greenhouse light intensity and quality on biomass and salidroside content in roots of Rhodiola sachalinensis

作　　者：阎秀峰[1] 王洋[1] 尚辛亥[1] 

机构地区：[1]东北林业大学森林资源与环境学院,哈尔滨150040

出　　处：《生态学报》2003年第5期841-849,共9页Acta Ecologica Sinica

基　　金：国家自然科学基金资助项目 (3 970 0 0 1 3 )~~

摘　　要：为探讨光强及光质对高山红景天 (Rhodiola sachalinensis)生物量和红景天甙含量的影响 ,于 2 0 0 0年 4月至 6月在东北林业大学温室内以移栽于大兴安岭加格达奇圃地人工种植生长 3 a的高山红景天为材料 ,通过纱布遮荫及遮以不同颜色的滤光膜分别进行了光强、光质控制实验 (处理 45 d)。随着光强的降低 ,高山红景天全株生物量、根生物量、根的红景天甙含量和产量以及叶中叶绿素 a、叶绿素 b、总叶绿素的含量均有降低的趋势 ,但叶绿素含量变化很小 ,不同光强及对照之间的差异均未达到显著水平。相对光强为 6 7.75 %和 44.71 %的两种处理下的全株生物量、根生物量、根的红景天甙含量和产量差异不显著 ,它们的全株生物量和红景天甙含量与对照 (全光照 )的差异也不显著 ,但根生物量和红景天甙产量与对照的差异显著。当相对光强减弱至 3 1 .96 % ,全株生物量、根生物量、根的红景天甙含量和产量均大幅度下降 ,根冠比显著增加。4种滤光膜处理均使高山红景天的全株生物量及根生物量显著降低 ,蓝膜和绿膜处理的降低幅度大于红膜和黄膜处理的。红膜处理的红景天甙的含量和产量均高于对照 ,但黄膜、蓝膜和绿膜处理的红景天甙含量和产量则低于对照。通过计算去除 4种滤光膜的光强因素 ,仅从光质的作用看 ,4种滤光膜?Rhodiola sachalinensis A. Bor, a perennial herb, belonging to the family Crassulaceae, is mainly distributed in mountains of 1700～2500 m above sea level. It is a very important medicinal plant with high activities of anti-fatigue, anti-senescence and anti-radiation, due to the secondary metabolite, salidroside in its roots. In order to investigate the effects of light intensity and light quality on plant growth and salidroside content in roots of Rh. sachalinensis, a controlled environmental study was conducted in a greenhouse in April 2000. Four light intensity levels (relative light intensities were 100%, 67.75%, 44.71% and 31.96% of full sunlight, respectively) were created by using neutral shading cloth, and four different light qualities were done by red, yellow, blue and green films (relative light intensities were 51.76%, 60.69%, 26.96% and 24.80% of full sunlight, respectively). On April 27, 3-year-old plants grown at a nursery in Daxinganling Mountain (124°02′E, 50°30′ N), were transplanted to pots (diameter 25 cm, depth 25 cm) in a greenhouse at Northeast Forestry University, Harbin, P. R. China (126°38′ E, 45°43′ N). Light controlled treatments were performed after 1 week of shoot development. Aboveground and root biomasses, the contents of chlorophyll a and chlorophyll b in leaves, and salidroside content in roots of Rh. sachalinensis were measured after 45 days of treatment, and whole plant biomass, root/shoot ratio, total chlorophyll content, chlorophyll a/b ratio and salidroside yield in roots (the product of salidroside content and root biomass) were calculated. Results revealed that the biomass of Rh. sachalinensis was affected by light intensity. The biomasses of the whole plant and roots tended to decrease as light intensity declined, though whole plant biomass was not influenced apparently when light intensity was 44.71% of full sunlight or higher. The biomasses of the whole plant were 87.82%, 84.95% and 37.46% with light intensities of 67.75%, 44.71% and 31.96% of full sun

关 键 词：温室栽培 光强 光质 高山红景天 生物量 红景天甙 含量 

分 类 号：Q945.79[生物学—植物学]