人子宫肌层诱导型环加氧酶剪接变异体cDNA片段的克隆和序列测定  

Expression of cyclooxygenase-2 mRNA and identification of its spliceriant in human myometrium obtained from women in labor

作　　者：黄引平[1] 叶笃筠[2] 吴萍[2] 黄云峰[2] 孙炜[2] 应红钢[2] 盘茜[2] 万敬员[2] 袁萍[2] 张代娟[2] 

机构地区：[1]温州医学院附属第一医院妇产科,325000 [2]华中科技大学同济医学院病理生理教研室

出　　处：《中华妇产科杂志》2003年第5期270-272,共3页Chinese Journal of Obstetrics and Gynecology

基　　金：国家自然科学基金资助项目 ( 30 0 70 92 9;30 2 7136 3)

摘　　要：目的　寻找人子宫肌层诱导型环加氧酶 (cyclooxygenase 2 ,COX 2 )选择性剪接变异体。方法　运用自行设计的COX 2mRNA引物 ,采用逆转录聚合酶链反应 (RT PCR)技术 ,分离临产与未临产产妇子宫肌层COX 2mRNA ,再对电泳所获条带进行亚克隆、测序。结果　未临产产妇子宫肌层未检测到COX 2mRNA ,临产产妇子宫肌层不仅检测到COX 2mRNA ,而且分离出一新条带 ,经亚克隆及测序 ,证实该条带系人子宫肌层COX 2蛋白DNA的第 7、8外显子间的内含子序列被保留所致。结论　临产产妇子宫肌层COX 2mRNA存在选择性剪接变异体。该变异体的发现 。Objective To investigate the expression of cyclooxygenase 2(COX 2) in human lower segments of myometrium obtained from women in labor and those not in labor and identify the splice variant of COX 2 Methods Reverse transcriptase polymerase chain reaction (RT PCR) was used for investigating the expression of COX 2.According to the sequence of rat COX 2 splice variant, the primers were designed and synthesized, then the splice variant of COX 2 in human myometrium from woman in labor was identified, cloned into vector and sequenced Results The results showed that (1) The Expression of COX 2 mRNA was lower in human myometrium obtained from women who were not in labor than those in labor.(2) A new band of COX 2 was obtained in myometrium from a woman in labor.The fragment includes an unspliced intron,which locates between exons 7 and 8 Conclusion COX 2 gene is not only expressed highly in human myometrium from women in labor, but also produced splicing variant by alternative splicing.

关 键 词：子宫肌层 诱导型环加氧酶 CDNA片段 克隆 序列测定 逆转录聚合酶链反应 分娩 前列腺素内过氧化物合酶 

分 类 号：R346[医药卫生—基础医学]