益肾活血方对家兔血瘀模型P选择素表达及对氧自由基清除作用的实验研究  

The Study of Yi-Shen-Huo-Xue Fang's Effects on Expression of GMP-140 and Cleaning Out the Oxygenic Free Radicle on Rabbits Blood Stasis Model

作　　者：詹小萍[1] 方青[2] 楼建国[1] 徐锡鸿 金晓莹[1] 秦光明[1] 鲍军[1] 

机构地区：[1]浙江大学医学院附属二院,浙江杭州310009 [2]浙江大学医学院附属邵逸夫医院,浙江杭州310016 [3]浙江中医研究院,浙江杭州300163

出　　处：《中国中药杂志》2003年第4期355-358,共4页China Journal of Chinese Materia Medica

基　　金：浙江省中医管理立项课题 ( 96 0 36 )

摘　　要：目的 :评价益肾活血方对家兔血瘀模型P选择素表达的影响以及清除氧自由基的作用。方法 :兔 30只 ,随机分为健康组、血瘀模型组、益肾活血方大剂量组和小剂量组、血栓心脉宁组 ,各组相应处理后分别取静脉血检测P选择素 (GMP 1 4 0 ) ,红细胞超氧化物歧化酶 (E SOD) ,红细胞过氧化脂质 (E LPO) ,血浆过氧化脂质 (P LPO)。结果 :GMP 1 4 0 ,E SOD ,E LPO ,P LPO ,健康组与血瘀模型组比P <0 .0 1 ,血瘀模型组与益肾活血方大、小剂量组比P <0 .0 1 ,与血栓心脉宁组比P <0 .0 5 ;益肾活血方大、小剂量组与血栓心脉宁组比P <0 .0 5 ;益肾活血方大剂量组与小剂量组比P >0 .0 5。结论 :造模成功 ,益肾活血方大、小剂量组及血栓心脉宁组均有抑制对家兔血瘀模型P选择素表达 ,增加SOD活性 ,降低LPO含量的作用。益肾活血方大。Objectve: To evaluate the effects of Yi Shen Huo Xue Fang on expression of GMP 140 and cleaning out the oxygenic free radicle on rabbits blood stasis model. Method: Thirty rabbits were divided randomly into five groups as the normal group, model group, large dose of 'Yi Shen Huo Xue Fang' group, small dose of “Yi Shen Huo Xue Fang' group and “Xue Shuan Xin Mai Ning' group. After being treated respectively , granule membrane protein 140(GMP 140),erythrocyte sueroxide dismutase (E SOD),erythrocyte lipid peroxide(E LPO),plasma lipid peroxide(P LPO) were checked up.Result: The GMP 140, E SOD, E LPO, P LPO in normal control were compared with those in model groups, With the difference( P <0.01),model control group was compared with large dose group and small dose group( P <0.01), with “Xue Shuan Xin Mai Ning'group( P <0.05), large dose group was compared with “Xue Shuan Xin Mai Ning' group( P <0.05),and large dose group were compared with small dose group( P >0.05).Conclusion:The model was made successfully. Large dose group, small dose group and “xue shuan xin mai ning'group can inhibit expression of GMP 140,enhence SOD activity and decrease LPO content on blood stasis rabbit model. Large dose group and small dose group have stronger effect than “xue shuan xin mai ning'group.[

关 键 词：血瘀症 益肾活血方 中药 P选择素 氧自由基 动物实验 

分 类 号：R285.5[医药卫生—中药学]