剪切力对南方红豆杉细胞悬浮培养的影响  

Effect of Shear Stress on Suspension Cultures of Taxus chinensis var. mairei

作　　者：韩荣斌[1] 施中东[1] 杨文莉[1] 元英进[1] 

机构地区：[1]天津大学化工学院制药工程系,天津300072

出　　处：《过程工程学报》2003年第2期135-140,共6页The Chinese Journal of Process Engineering

基　　金：国家自然科学基金资助项目(编号:20176038)

摘　　要：考察了流体剪切力对南方红豆杉悬浮细胞线粒体活性、膜透性、培养液pH、酚积累以及氧迸发等的影响. 结果表明,指数后期或稳定前期的南方红豆杉细胞对剪切力更为敏感,细胞生物活性丧失较大,次生代谢物酚的积累更多;此外,剪切力还引起了红豆杉细胞的防御应答反应,如H+内流、酚类物质大量积累以及活性氧迸发.Defined laminar shear field was created for the suspension cultures of Taxus chinensis var. mairei in a Couette-type shear reactor. It was found that the suspension cultures in the latter stage of exponential growth and early in the stationary phase were more susceptible to shear damage than cultures in the lag phase and early exponential phase, resulting in the most loss of the cell viability and the most accumulation of phenolics. Additionally, the shear stress caused the defense responses of Taxus chinensis var. mairei such as influx of H+, accumulation of phenolics and oxidative burst.

关 键 词：南方红豆杉细胞 悬浮培养 剪切力 杉细胞防御应答反应 

分 类 号：Q942.6[生物学—植物学]