BRM释介素抗肿瘤作用研究  

Antitumor effect research of BRM

作　　者：刘朝阳[1] 王泽光 田海梅[1] 娜迪娅 王宇部 曹冬艳[1] 刘毅[1] 李茉[1] 梁军林[1] 张伟[1] 

机构地区：[1]中国医学科学院,中国协和医科大学肿瘤研究所,北京100021 [2]北京临床肿瘤基因研究所

出　　处：《肿瘤防治研究》2003年第3期206-209,共4页Cancer Research on Prevention and Treatment

摘　　要：目的　研究中药复方胶囊BRM释介素的抗癌活性及诱导人神经胶质瘤细胞SHG 4 4凋亡。方法　运用MTT法检测BRM对体外人癌细胞株的抑制作用 ;运用小鼠移植瘤模型观察BRM体内抑瘤活性 ;应用电镜、流式细胞仪分析、琼脂糖凝胶电泳观察BRM诱导SHG 4 4。结果　BRM释介素体外对SHG 4 4、MCF 7具有明显的抑制作用 ;体内对小鼠移植性肿瘤表现出较显著的抗癌活性 ;SHG 4 4在BRM水提液作用下发生细胞凋亡。Objective To evaluate the antitumor action of BRM, and its mechanism.Methods Inhibition tumor experiment study on mice which was transplant tumor cell strains in mice subcutaneous tissue in vivo and MTT method tested human tumor cell lines in vitro and tumor cells include cell electric morphology, flow cytometry, agarose gel electrophoresis, were performed for determining apoptosis in tumor cells.Results It was found that BRM had a significant inhibition rate of tumor reproduce in vivo and in vitro. Meanwhile BRM was able to induce apoptosis in SHG 44 cells strain, marked morphological changes were observed with electric microscope, agarose gel electrophoresis of DNA from SHG 44 cells treated with BRM extracts( 1.25 mg to 5mg)for 24h, 40h revealed 'Ladder' pattern. The early stage and middlelate stage apoptosis increased in SHG 44 cells treated with BRM extracts(BRM 0.62 mg to 2.5 mg) from 14h to 48h by Annexin V/PI flow cytometry analysis.Conclusion BRM have obvious antitumor activity to MPC 83,EAC, S 180,in vivo and SHG 44, MCF 7 in vitro and water extracts of BRM could induce apoptosis of SHG 44 cells Line.

关 键 词：BRM释介素 中药制剂 抗肿瘤作用 细胞凋亡 

分 类 号：R273[医药卫生—中西医结合]