棉疫病菌培养液中激发子活性成分的分析  

The elicitor components in culture liquid of Phytophthora boehmeriae

作　　者：王源超[1] 胡东维[2] 张正光[1] 马志超[2] 

机构地区：[1]南京农业大学农业部病虫监测与治理重点开放实验室,江苏南京210095 [2]浙江大学生物技术研究所,浙江杭州310029

出　　处：《南京农业大学学报》2003年第2期38-41,共4页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金资助项目 (3 980 0 0 0 6;3 9970 478)

摘　　要：棉疫病菌 (Phytophthoraboehmeriae)的培养滤液经饱和硫酸铵沉淀后 ,经 30× 10 3 超滤膜进行分子量截留 ,将大于和小于 30× 10 3 的蛋白组分分别处理棉疫病菌的非寄主植物烟草。结果发现 ,两组分均能引起烟草叶片的过敏性坏死反应。提示在棉疫病菌培养液中至少存在两类不同分子量的激发子成分。对大于 30× 10 3 组分中可能存在的激发子的性质进行分析。结果发现 ,经 10 0℃处理 5min后 ,该组分的激发子仍能保持其生物学活性 ;采用蛋白酶K处理可使该组分丧失激发子活性 ;该激发子经 10 0 0倍稀释后处理烟草 ,仍可诱导烟草的过敏性坏死反应 ,并可强烈诱导烟草对烟草花叶病毒的系统抗病性 ;该激发子在棉疫病菌培养液中培养 10d左右可达最大积累量。以上结果表明 ,在棉疫病菌的培养液中至少还存在一种新的相对分子质量大于 30× 10 3 的蛋白质激发子 ,该激发子热稳定 ,其活性基团位于蛋白质部分。The cultured filtrate of Phytophthora boehmeriae was saturated by(NH 4) 2 SO 4,ultrafiltrating the culture filtrate with 30× 10 3 molecular weight membrane yielded two fractions.Both fractions caused hypersensitive necrosis on tobacco leaves,it was suggested that P.boehmeriae could secrete at least two kinds of protein elicitors,with different molecular weight.The protein elicitor with molecular weight larger than 30×10 3 was given an intensively study in this work.Boiled in 100 ℃ water for 5 min,the elicitor still could induce HR in tobacco;Treated with protease K,the elicitor lost its activity,that means the protein domain is the activity part;After diluted about 1 000 times,the elicitor could still induce tobacco resistance to TMV about 80%.It was indicated that, P.boehmeriae could secrete a novel protein elicitor with molecular weight larger than 30×10 3,which could induce HR in tobacco and its activity was determined by protein domain.A highest protein accumulation was given after ten days′ culture.

关 键 词：棉疫病菌 培养液 激发子 棉花 病原菌 分子质量组分 活性成分 抗病性 

分 类 号：S435.621.2[农业科学—农业昆虫与害虫防治]