黄芩苷对脂多糖诱导牙周膜成纤维细胞表达炎症因子的影响  

Effects of baicalin on the expression of inflammatory cytokines in human periodontal ligament fibroblasts induced by lipopolysaccharide

作　　者：孙丽君 周洪[2] 张晨[3] 何峰[4] 温爽[2] 胡佳艺[1] 居文正[1] 戴国梁[1] 束为[1] Sun Lijun;Zhou Hong;Zhang Chen;He Feng;Wen Shuang;Hu Jiayi;Ju Wenzheng;Dai Guoliang;Shu Wei(Department of Stomatology,Jiangsu Provincial Hospital of Traditional Chinese Medicine,Nanjing 210029;Department of Immunology,NMU,Nanjing 211166;Department of Biomedical Research,the Second Affiliated Hospital of NMU,Nanjing 210011;Department of Speciality,Affiliated Stomatological Hospital of NMU,Nanjing 210029,China)

机构地区：[1]江苏省中医院口腔科,江苏南京210029 [2]南京医科大学免疫学系,江苏南京211166 [3]南京医科大学第二附属医院生物医学研究室,江苏南京210011 [4]南京医科大学附属口腔医院特诊科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2019年第1期50-53,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省中医院科技发展基金(Y16020)

摘　　要：目的:探讨黄芩苷对脂多糖(lipopolysaccharide,LPS)促人牙周膜成纤维细胞炎症因子表达的影响。方法:培养后的人牙周膜成纤维细胞用不同浓度的黄芩苷干预,CCK8实验观察黄芩苷对人牙周膜成纤维细胞的细胞增殖毒性。培养后的人牙周膜成纤维细胞分为空白对照组、黄芩苷组、LPS组和LPS+黄芩苷组。空白对照组仅加入2%胎牛血清的培养液;黄芩苷组分别加入黄芩苷200、500 ng/mL;LPS组加入100μg/mL LPS;LPS+黄芩苷组加入100μg/mL LPS,同时分别加入黄芩苷200、500 ng/mL。24 h收集细胞,real-time PCR检测各组白介素(interleukin,IL)-6、IL-8、IL-1βmRNA表达的变化。结果:CCK8实验结果显示,500 ng/mL浓度以下黄芩苷对细胞没有明显的增殖毒性。和空白对照组比较,黄芩苷组(200 ng/mL和500 ng/mL)IL-6、IL-8、IL-1β的mRNA表达均无明显变化(P>0.05),LPS组IL-6、IL-8、IL-1βmRNA表达明显上升,差异有统计学意义(P <0.05);相比LPS组,LPS+黄芩苷(200 ng/mL)组IL-6、IL-8、IL-1βmRNA表达明显下降,差异有统计学意义(P <0.05),而LPS+黄芩苷(500 ng/mL)组IL-6、IL-1βm RNA表达明显上升,差异有统计学意义(P <0.05)。结论:黄芩苷在低浓度时显示有抑炎作用,而在浓度增加达到500 ng/mL时显示有促炎作用。Objective:To investigate the effect of baicalin on the expression of inflammatory cytokines in human periodontal ligament fibroblasts(HPDLFs)induced by lipopolysaccharide(LPS). Methods:After the cultured HPDLFs were treated with different concentrations of baicalin,the cytotoxicity of different concentrations of baicalin on HPDLFs was observed by CCK8. The cultured HPDLFs were divided into the blank control group,the baicalin group,the LPS group,and the LPS + baicalin group. The blank control group only added 2% fetal bovine serum culture solution;the baicalin group added 200 or 500 ng/mL baicalin,respectively;the LPS group added 100 μg/mL LPS;the LPS + baicalin group added 100 μg/mL LPS,and 200 or 500 ng/mL baicalin,respectively. Cells were collected 24 h later. The changes of IL-6,IL-8,and IL-1β mRNA expressions in each group were examined. Results:The results of the CCK8 experiment show that the addition of baicalin to the cells at a concentration of 500 ng/mL had no significant cell proliferation toxicity. There was no significant change in the expression of IL-6,IL-8 and IL-1βcompared with the baicalin group of baicalin 200 ng/m L and 500 ng/mL(P>0.05). Compared with the blank control group,the expression of IL-6,IL-8 and IL-1β in the LPS group increased significantly(P < 0.05). At the same time,when LPS + 200 ng/mL baicalin were added,the expression of IL-6,IL-8 and IL-1β were significantly decreased compared with LPS alone(P < 0.05). When LPS + 500 ng/mL baicalin were added,the expression of IL-6,and IL-1β were significantly increased compared with LPS alone(P < 0.05). Conclusion:Baicalin showed anti-inflammatory effect at a low concentration,and showed pro-inflammatory effect when the concentration increased to 500 ng/mL.

关 键 词：黄芩苷 脂多糖 人牙周膜成纤维细胞 

分 类 号：R285.5[医药卫生—中药学]