IL-10对人肾小球系膜细胞细胞周期负调控蛋白p27的调节  

Effects of IL-10 on the Cell Cycle Negative Regulatory Protein p27 in Human Glomerular Mesangial Cells

作　　者：吴元俊[1] 陈吉庆[1] 唐云章[1] 张爱华[2] 陈荣华[2] 

机构地区：[1]南京医科大学第一附属医院儿科,南京210029 [2]南京医科大学小儿肾脏病研究中心,南京210029

出　　处：《上海免疫学杂志》2003年第3期155-157,共3页Shanghai Journal of Immunology

基　　金：江苏省教委基金资助项目 (No 0 0KJB3 2 0 0 0 6)

摘　　要：观察白细胞介素 10 (IL 10 )对体外培养的人肾小球系膜细胞 (HMC )增殖影响 ,并探讨IL 10对HMC细胞周期负调控蛋白p2 7的影响 ,旨在了解其调节HMC增殖的内在机制。HMC用含 5 %FCS的RPMI16 4 0培养 ,加入IL 10刺激后 ,应用流式细胞术分别测定HMC细胞增殖周期的变化以及p2 7的水平。结果表明IL 10可抑制血清诱导的HMC增殖 ,IL 10显著减少了HMCG0 /G1期细胞进入S期和G2 /M期 ;2 5ng/mlIL 10明显地上调细胞周期负调控蛋白p2 7水平 ,是血清刺激组的 1 7倍 (P <0 0 5 )。IL 10抑制血清诱导的HMC增殖的作用机制可能部分是通过上调细胞周期负调控蛋白p2 7来实现的 ,提示ILTo observe the effects of IL 10 on the proliferation of human glomerular mesangial cells and the cell cycle negative regulatory protein p27,the glomerular mesangial cells were cultivated in RPMI 1640 supplemented with 5% FCS in the presence or absence of 25 ng/ml of IL 10 Flow cytometry was used to analyze the proliferation of mesangial cells and to determine the level of p27 It was found that IL 10 could inhibit the proliferation of these cells in vitro and remarkably reduce the numbers of mesangial cells from G0/G1 phase of cell cycle entering into S and G2/M phase,thus suppressing the proliferation of mesangial cells After treatment wih IL 10,the level of p27 was up regulated to 1 7 times It concludes that the effects of IL 10 to inhibit proliferation of mesangial cells may be mainly through the regulation of the cell cycle protein p27 and IL 10 may play an important role in the pathogenesis of prolifrative glomerulonephritis

关 键 词：白细胞介素10 人系膜细胞 增殖 P27 

分 类 号：R392.12[医药卫生—免疫学]